Cardiovascular Disease

Photomed Laser Surg. 2016 Sep 14. [Epub ahead of print]

Low-Level Laser Irradiation Precondition for Cardiac Regenerative Therapy.

Liu Y1, Zhang H1.

Author information

  • 1State Key Laboratory of Cardiovascular Disease and Key laboratory of Cardiac Regenerative Medicine, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing, China .



The purpose of this article was to review the molecular mechanisms of low-level laser irradiation (LLLI) preconditioning for heart cell therapy.


Stem cell transplantation appears to offer a better alternative to cardiac regenerative therapy. Previous studies have confirmed that the application of LLLI plays a positive role in regulating stem cell proliferation and in remodeling the hostile milieu of infarcted myocardium. Greater understanding of LLLI’s underlying mechanisms would be helpful in translating cell transplantation therapy into the clinic.


Studies investigating LLLI preconditioning for cardiac regenerative therapy published up to 2015 were retrieved from library sources and Pubmed databases.


LLLI preconditioning stimulates proliferation and differentiation of stem cells through activation of cell proliferation signaling pathways and alteration of microRNA expression. It also could stimulate paracrine secretion of stem cells and alter cardiac cytokine expression in infarcted myocardium.


LLLI preconditioning provides a promising approach to maximize the efficacy of cardiac cell-based therapy. Although many studies have reported possible molecular mechanisms involved in LLLI preconditioning, the exact mechanisms are still not clearly understood.

Medicine (Baltimore). 2015 Oct;94(42):e1724.

Periodontal Treatment Elevates Carotid Wall Shear Stress in the Medium Term.

Carallo C1, Franceschi MS, Tripolino C, Iovane C, Catalano S, Giudice A, Crispino A, Figliuzzi M, Irace C, Fortunato L, Gnasso A.
Author information
1From the Department of Chemical Engineering, Imperial College London, London, United Kingdom (CC, SC); Department of Clinical and Experimental Medicine, Institute of Dentistry, “Magna Graecia” University (MSDF, CI, AG, AC, MF, LF); and Department of Clinical and Experimental Medicine, Metabolic Disease Unit, “Magna Graecia” University, Catanzaro, Italy (CC, MSDF, CT, CI, AG).
Periodontal disease is associated with endothelial dysfunction of the brachial artery and hemodynamic alterations of the common carotid artery. Periodontal therapy improves endothelial function. It is not known if it is able also to improve the hemodynamics of the carotid artery. The aim of the current study was to evaluate the efficacy of 2 different periodontal treatments on carotid hemodynamics: scaling and root planing (SRP) alone or together with low-level laser therapy (LLLT). Forty patients were recruited and randomly treated with SRP (n=20) or SRP + LLLT (n=20). Periodontal indices (plaque, gingival, and probing depth indices) were measured before and 5 months after treatment. Blood viscosity, common carotid wall shear stress, circumferential wall tension, and Peterson elastic modulus were evaluated before, soon after and 5 months after treatment. It was found that the periodontal indices improved in both groups, but significantly more so for SRP + LLLT than for SRP (decrease in gingival index 69.3% versus 45.4%, respectively, P=0.04). In the SRP + LLLT group, after a transient reduction by 5% immediately after therapy, shear stress increased by 11% after 5 months. In SRP only group, however, shear stress variations were less marked. No significant changes were found for the other hemodynamic parameters in either of the groups. Periodontal disease treatment by SRP + LLLT can therefore be said to improve common carotid wall shear stress. This suggests a possible mechanism by which the treatment of periodontal disease has beneficial effects on the cardiovascular system.
Photomed Laser Surg. 2014 Oct;32(10):582-587.

Effects of Intravascular Low-Level Laser Therapy During Coronary Intervention on Selected Growth Factors Levels.

Derkacz A1, Protasiewicz M, Rola P, Podgorska K, Szymczyszyn A, Gutherc R, Por?ba R, Doroszko A.

Author information

  • 11 Department of Internal Medicine and Hypertension, Wroclaw Medical University , Wroclaw, Poland .


Abstract Objective: The objective of this study was to evaluate the effect of intravascular low-level laser therapy (LLLT) on selected growth factor levels in subjects undergoing percutaneous coronary interventions (PCI).

Background data: Restenosis remains the main problem with the long-term efficacy of PCI, and growth factors are postulated to play a crucial role in the restenosis cascade.

Materials and methods: In a randomized prospective study, an 808?nm LLLT (100?mW/cm2, continuous wave laser, 9?J/cm2, illuminated area 1.6-2.5?cm2) was delivered intracoronarily to patients during PCI. Fifty-two patients underwent irradiation with laser light, and 49 constituted the control group. In all individuals, serum levels of insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), transforming growth factor-?1 (TGF-?1), and fibroblast growth factor-2 (FGF-2) were measured before angioplasty, then 6 and 12?h and 1 month after the procedure. In all patients, a control angiography was performed 6 months later.

Results: There were no significant differences in IGF-1 and VEGF levels between the groups. While evaluating FGF-2, we observed its significantly lower levels in the irradiated patients during each examination. There was a significant increase in TGF-?1 level in control group after 12?h of observation. In the irradiated individuals, control angiography revealed smaller late lumen loss and smaller late lumen loss index as compared with the control group. The restenosis rate was 15.0% in the treated group, and 32.4% in the control group, respectively.

Conclusions: LLLT decreases levels of TGF-?1 and FGF-2 in patients undergoing coronary intervention, which may explain smaller neointima formation.

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This Journal Search Submit a Manuscript Arquivos Brasileiros de Cardiologia
Arq Bras Cardiol. 2014 Aug; 103(2): 161–164.
doi:  10.5935/abc.20140117
PMCID: PMC4150668

Hemodynamic Effect of Laser Therapy in Spontaneously Hypertensive Rats

Suely Tomimura,1 Bianca Passos Assumpção Silva,2 Iris Callado Sanches,3 Marina Canal,2 Fernanda Consolim-Colombo,2,4 Felipe Fernandes Conti,3 Katia De Angelis,3 and Maria Cristina Chavantes1,2,4
1Programa de Pós-Graduação em Biofotônica em Ciências da Saúde da Universidade Nove de Julho (UNINOVE)
2Programa de Graduação e Pós-Graduação em Medicina da UNINOVE
3Laboratório de Fisiologia Translacional da UNINOVE
4Unidade de Hipertensão e Central Médica de Laser do Instituto do Coração – Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo (InCor/HC-FMUSP), São Paulo, SP ? Brazil
Mailing Address: Suely Tomimura, Av. Santa Inês, 1.129, Alto do Mandaqui. Postal Code 02415-001, São Paulo, SP ? Brazil. E-mail: rb.moc.lou@arumimotyleus
Received 2014 Mar 2; Revised 2014 Apr 28; Accepted 2014 May 19.


Systemic arterial hypertension (SAH) is considered to be the greatest risk factor for the development of neuro-cardiovascular pathologies, thus constituting a severe Public Health issue in the world.

The Low-Level Laser Therapy (LLLT), or laser therapy, activates components of the cellular structure, therefore converting luminous energy into photochemical energy and leading to biophysical and biochemical reactions in the mitochondrial respiratory chain. The LLLT promotes cellular and tissue photobiomodulation by means of changes in metabolism, leading to molecular, cellular and systemic changes.

The objective of this study was to analyze the action of low-level laser in the hemodynamic modulation of spontaneously hypertensive rats, in the long term. Animals (n = 16) were randomly divided into the Laser Group (n = 8), which received three weekly LLLT irradiations for seven weeks, and into the Sham Group (n = 8), which received three weekly simulations of laser for seven weeks, accounting for 21 applications in each group. After seven weeks, animals were cannulated by the implantation of a catheter in the left carotid artery. On the following day, the systemic arterial pressure was recorded. The Laser Group showed reduced levels of mean blood pressure, with statistically significant reduction (169 ± 4 mmHg* vs. 182 ± 4 mmHg from the Sham Group) and reduced levels of diastolic pressure (143 ± 4 mmHg* vs. 157 ± 3 mmHg from the Sham Group), revealing a 13 and 14 mmHg decrease, respectively. Besides, there was a concomitant important decline in heart rate (312 ± 14 bpm vs. 361 ± 13 bpm from the Sham Group). Therefore, laser therapy was able to produce hemodynamic changes, thus reducing pressure levels in spontaneously hypertensive rats.

Keywords: Low-Level Laser Therapy (LLLT), Experimental Laser Therapy, Hypertension, Hemodynamic Changes

Hypertension is the causa mortis of 9.5 million people around the world1, constituting a major Public Health issue.

The LLLT, or laser therapy, is able to induce a photobiological response inside the cells, activating the production of Adenosine Triphosphate (ATP), Nitric Oxide (NO) and Reactive Oxygen Species (ROS); it also changes sodium-potassium pumps and calcium channels also facilitate membrane permeability2.

According to Chavantes and Tomimura3, laser therapy reduces the inflammatory and edematous process, and it also changes the micro and macrovascular response, assisting in tissue repair and enabling analgesia. LLLT has proven to be an efficient, non-invasive, low-cost and safe tool.

A pioneer experimental work4 analyzed the acute short term actions of LLLT (only three applications) on Systemic Blood Pressure (SBP) in obese and old Wistar rats. The results indicated that LLLT was able to significantly decrease pressure levels, thus revealing cardiovascular protection, which guided the study proposed in this paper.

Therefore, the objective of this study was to evaluate the long term effects of LLLT on the hemodynamic response of Spontaneously Hypertensive Rats (SHR), by assessing Systolic Blood Pressure (SBP), Diastolic Blood Pressure (DBP), Mean Blood Pressure (MBP) and Heart Rate (HR).


A prospective, controlled experimental study with 16 male SHR, which were randomly divided into two groups (n = 8): Sham Group and Laser Group. Three weekly LLLT applications were conducted on alternate days, for 7 weeks, accounting for 21 applications in the Laser group. In the Sham Group, the same protocol was implemented, however, the equipment was turned off.

The sample size and the method were based on studies found in literature, assessing hemodynamic changes and physical condition in SHR animals5,6.

The laser diode (MMOptics) was transcutaneously applied on the rats’ tails, as demonstrated in figures 1 and ?and2,2, with the following parameters: wavelength (?) = 780 nm, flow = 30 J/cm2, power = 40 mW, spot size = 0.04cm2 and irradiance = 1W/cm2, accounting for 90 seconds.

Figure 1

Laser diode 780 nm (MMOptics, São Carlos, SP, Brazil).

Figure 2

Place of irradiation: dorsal tail.

After seven weeks, all animals were anesthetized and cannulated by a catheter implantation in the left carotid artery. After the implantation, they were passed subcutaneously and exteriorized in the cervical dorsal region. After the animal had woken up, after 24 hours, the arterial cannula was attached to an electromagnetic transducer (Blood Pressure XDCR, Kent© Scientific, Litchfield, CT, USA) and to the pre-amplifier (Stemtech BPMT-2, Quintron Instrument© Inc, Milwaukee, USA). Signs of Blood Pressure (BP) were digitally recorded for 30 minutes, by means of a data acquisition system (CODAS, 1Kz, Dataq Instruments, Akron, OH, USA). This enabled the analysis of pressure pulse, beat by beat, with sampling frequency of 2,000 Hz per channel, for the study of SBP, DBP, MBP and HR. HR values were derived from the pulse signal of BP7.

Statistical analyses

Values are presented as means ± standard deviations of means. HR, MBP, SBP and DBP were compared between the Sham and the Laser Groups. After the evaluation of distributions by the Kolgomorov normality test, the Student’s t-test was used to verify the differences between normal distributions. The adopted statistical software was the GraphPad InStat. Significance level was established as p < 0.05.


The Laser Group showed reduction in relation to the Sham Group in the following values: MBP (169 ± 4 mmHg* vs. 182 ± 4 mmHg from the Sham Group) and DBP (143 ± 4 mmHg* vs. 157 ± 3 mmHg from the Sham Group), presenting statistically significant differences. The SBP value (196 ± 5 mmHg vs. 207 ± 4 mmHg from the Sham Group) revealed no differences, as demonstrated in Table 1.

Table 1

Hemodynamic parameters assessed at rest in the Laser and Sham groups

With regard to HR at rest, there was significant decline in heart beats in the Laser Group (Figure 3) when compared to the Sham Group (312 ± 14* bpm vs. 361 ± 13 bpm in the Sham Group).

Figure 3

Diastolic Blood Pressure (DBP), Systolic Blood Pressure (SBP), mean blood pressure (MBP) and heart rate (HR) of Laser and Sham Groups. * p < 0.05.


Nowadays, SAH is one of the most prevalent causa mortis. Therapeutic strategies that aim at the reduction of SBP are considered to be important.

Nowadays, LLLT is a relevant instrument in the therapeutic arsenal of numberless health fields, being able to modulate the genic expression of chemokines, to change cytokines and NO synthetic inducers. These changes may have important therapeutic relevance in vascular inflammatory processes8.

The endothelial system plays an essential role to control muscular tonus, responding to dynamic changes in blood flow (shear stress). Regular physical activities are able to stimulate vasodilating factors, thus stimulating the liberation of factors such as NO and the hyperpolarizing factor derived from the endothelium, thus reducing BP levels9.

In an in vitro experiment, Ricci10 demonstrated that endothelial cells submitted to nutritional stress effectively respond to LLLT irradiation, reorganizing actin filaments in the cytoskeleton, associated with endothelial/cellular proliferation.

A study involving the physical conditioning of SHR demonstrated pressure reduction post-physical training, which led to an important HR decline and, consequently, to decreasing cardiac output11.

Our experiment observed that HR decreased relevantly in the Laser Group in relation to the Sham group (312 ± 14 bpm vs. 361 ± 13 bpm), which shows this is one of the possible mechanisms that are able to decrease cardiac output, and, consequently, SAH.

Sanches et al5, in another experiment with oophorectomized hypertensive female rats (during menopause), used physical training for 8 weeks and demonstrated that physical exercise decreased levels of DBP, SBP, MBP and HR when compared to sedentary hypertensive female rats.

Our study showed expressive decline after LLLT irradiation in the long term, with regard to baseline SHR pressure levels. Both the levels of DBP and MBP were reduced post laser therapy (7 weeks), with statistically significant differences between groups. There are other ongoing experiments in order to understand the mechanisms involved in SAH versus LLLT.


Laser therapy applied on spontaneously hypertensive rats in the long term resulted in reduced pressure levels, therefore modulating, expressively, the hemodynamic response among hypertensive rats.


Contributed by

Author contributions

Conception and design of the research: Tomimura S, Sanches IC, Chavantes MC; Acquisition of data: Tomimura S, Silva BPA, Canal M, Conti FF; Analysis and interpretation of the data: Tomimura S, Sanches IC, Conti FF, De Angelis K, Chavantes MC; Statistical analysis: Tomimura S, Sanches IC; Writing of the manuscript: Tomimura S; Critical revision of the manuscript for intellectual content: Sanches IC, Consolim-Colombo F, De Angelis K, Chavantes MC.

Potential Conflict of Interest

No potential conflict of interest relevant to this article was reported.

Sources of Funding

There were no external funding sources for this study.

Study Association

This article is part of the thesis of master submitted by Suely Tomimura, from Universidade Nove de Julho.:


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4. Canal M, Conti FF, Sanches IC, Pinto N, Pinto M, Silva B. Hemodynamic changes in elderly obese rats after low level laser therapy: an experimental study [abstract] American Society for Laser Medicine and Surgery Abstracts; Boston: 2013. p. 52.
5. Sanches IC, de Oliveira Brito J, Candido GO, da Silva Dias D, Jorge L, Irigoyen MC, et al. Cardiometabolic benefits of exercise training in an experimental model of metabolic syndrome and menopause. Menopause. 2012;19(5):562–568. [PubMed]
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PLoS One. 2014 Jul 3;9(7):e101270. doi: 10.1371/journal.pone.0101270. eCollection 2014.

Amelioration of Cardiac Function and Activation of Anti-Inflammatory Vasoactive Peptides Expression in the Rat Myocardium by Low Level Laser Therapy.

Manchini MT1, Serra AJ1, Feliciano RD1, Santana ET1, Antônio EL2, de Tarso Camillo de Carvalho P1, Montemor J2, Crajoinas RO3, Girardi AC3, Tucci PJ2,Silva JA Jr1.

Author information

  • 1Universidade Nove de Julho, UNINOVE, São Paulo, SP, Brasil.
  • 2Universidade Federal de São Paulo, UNIFESP, São Paulo, SP, Brasil.
  • 3Heart Institute (InCor), Universidade de São Paulo, USP, São Paulo, SP, Brasil.



Low-level laser therapy (LLLT) has been used as an anti-inflammatory treatment in several disease conditions, even when inflammation is a secondary consequence, such as in myocardial infarction (MI). However, the mechanism by which LLLT is able to protect the remaining myocardium remains unclear. The present study tested the hypothesis that LLLT reduces inflammation after acute MI in female rats and ameliorates cardiac function. The potential participation of the Renin-Angiotensin System (RAS) and Kallikrein-Kinin System (KKS) vasoactive peptides was also evaluated. LLLT treatment effectively reduced MI size, attenuated the systolic dysfunction after MI, and decreased the myocardial mRNA expression of interleukin-1 beta and interleukin-6 in comparison to the non-irradiated rat tissue. In addition, LLLT treatment increased protein and mRNA levels of the Mas receptor, the mRNA expression of kinin B2 receptors and the circulating levels of plasma kallikrein compared to non-treated post-MI rats. On the other hand, the kinin B1 receptor mRNA expression decreased after LLLT. No significant changes were found in the expression of vascular endothelial growth factor (VEGF) in the myocardial remote area between laser-irradiated and non-irradiated post-MI rats. Capillaries density also remained similar between these two experimental groups. The mRNA expression of the inducible nitric oxide synthase (iNOS) was increased three days after MI, however, this effect was blunted by LLLT. Moreover, endothelial NOS mRNA content increased after LLLT. Plasma nitric oxide metabolites (NOx) concentration was increased three days after MI in non-treated rats and increased even further by LLLT treatment. Our data suggest that LLLT diminishes the acute inflammation in the myocardium, reduces infarct size and attenuates left ventricle dysfunction post-MI and increases vasoactive peptides expression and nitric oxide (NO) generation.

Vopr Kurortol Fizioter Lech Fiz Kult. 2014 Jul-Aug;(4):3-6.

The application of chromo- and laserotherapy for the treatment of the patients presenting with chronic obstructive pulmonary disease and concomitant arterial hypertension.

[Article in Russian]
Nikitin AV, Marks SI.


This article gives evidence of the effectiveness of chromo- and laser therapy (using infrared and green wavelenth radiation) in combination with basal pharmacotherapy in the patients presenting with combined pathology. The analysis of the data obtained indicates that the proposed approach makes it possible to accelerate normalization of the clinical characteristics, reduce arterial pressure, improve the parameters of external respiration, and increase the duration of remission periods.




Photomed Laser Surg.  2013 Sep;31(9):447-52. doi: 10.1089/pho.2013.3481.




Effect of low-level laser irradiation on oxygen free radicals and ventricular remodeling in the infarcted rat heart.

Yang J, Huang Z, Zhou Y, Sai S, Zhu F, Lv R, Fa X.


1 Department of Cardiovascular Surgery, the Second Affiliated Hospital of Zhengzhou University , Zhengzhou, People’s Republic of China .




Abstract Objective: The purpose of this study was to assess the effects of low-level laser irradiation (LLLI) on the expression of oxygen free radicals (OFR) and ventricular remodeling (VR) in the model of rat myocardial infarction (RMMI).

Background data: LLLI reduces the infarct size and formation of scar tissue in the rat heart after myocardial infarction (MI). However, the exact mechanism has not been demonstrated so far.

Methods: RMMI was induced by ligating the left anterior descending coronary artery (LAD). After 3 weeks, LLLI (635 nm, 6 mW laser, 7.64 mW/cm(2), 125 sec, 0.96J/cm(2)) was applied to the surface of heart directly. Four to six rats were euthanized at 1h, 24h, 48h, 72h, and 1 week after LLLI, and the infarcted myocardia were excised for the measurement of the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA). At the end of 4 weeks after MI, the hearts were harvested for histological analysis.

Results: Myocardial SOD activity with LLLI was lower compared with control (p<0.05), and myocardial MDA content with LLLI was higher compared with control (p<0.05), at all the time points. In all rats, the activity of SOD was down to the minimum and the content of MDA was up to the peak at 48h after laser irradiation. The infarct size was reduced (35±10% vs. 18±9%, p<0.05), the left ventricular wall thickness was increased (0.31±0.03 vs. 0.84±0.02?mm, p<0.05) and the percentage of collagen fibers in the infarcted area was attenuated (64.34±2.20% vs. 30.97±2.60%) by LLLI.

Conclusions: LLLI could cause OFR accumulation, reduce infarct size, increase ventricular wall thickness, and attenuate the formation of collagen fibers, suggesting the beneficial effects of LLLI on improvement of VR after MI.





J Mol Cell Cardiol 2013 May 20

Far red/near infrared light treatment promotes femoral artery collateralization in the ischemic hindlimb.

Lohr NL, Ninomiya JT, Warltier DC, Weihrauch D

Department of Medicine, Division of Cardiovascular Medicine, Medical College of Wisconsin, USA; Department of Cardiovascular Center, Medical College of Wisconsin, USA; Clement J Zablocki VA Medical Center, USA. Electronic address:

Nitric oxide (NO) is a crucial mediator of hindlimb collateralization and angiogenesis. Within tissues there are nitrosyl-heme proteins which have the potential to generate NO under conditions of hypoxia or low pH. Low level irradiation of blood and muscle with light in the far red/near infrared spectrum (670nm, R/NIR) facilitates NO release. Therefore, we assessed the impact of red light exposure on the stimulation of femoral artery collateralization. Rabbits and mice underwent unilateral resection of the femoral artery and chronic R/NIR treatment. The direct NO scavenger carboxy-PTIO and the nitric oxide synthase (NOS) inhibitor L-NAME were also administered in the presence of R/NIR. DAF fluorescence assessed R/NIR changes in NO levels within endothelial cells. In vitro measures of R/NIR induced angiogenesis were assessed by endothelial cell proliferation and migration. R/NIR significantly increased collateral vessel number which could not be attenuated with L-NAME. R/NIR induced collateralization was abolished with c-PTIO. In vitro, NO production increased in endothelial cells with R/NIR exposure, and this finding was independent of NOS inhibition. Similarly R/NIR induced proliferation and tube formation in a NO dependent manner. Finally, nitrite supplementation accelerated R/NIR collateralization in wild type C57Bl/6 mice. In an eNOS deficient transgenic mouse model, R/NIR restores collateral development. In conclusion, R/NIR increases NO levels independent of NOS activity, and leads to the observed enhancement of hindlimb collateralization.

Lasers Med Sci.  2012 Aug 31. [Epub ahead of print]

Low-level laser therapy improves the inflammatory profile of rats with heart failure.

Hentschke VS, Jaenisch RB, Schmeing LA, Cavinato PR, Xavier LL, Dal Lago P.


Laboratório de Fisiologia, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil.


Following heart failure (HF), immune activation leads to an imbalance between pro-inflammatory and anti-inflammatory cytokines. Low-level laser therapy (LLLT) has been used as an anti-inflammatory treatment in several disease conditions. However, the effect of LLLT on the skeletal muscle of rats with HF remains unclear. The present report aimed to evaluate the influence of LLLT on the inflammatory profile of rats with HF. The left coronary artery was ligated to induce HF and a sham operation was performed in the control groups. Male Wistar rats (n=49) were assigned to one of six groups: placebo sham rats (P-Sham; n=8), LLLT at a dose of 3 J/cm(2) sham rats (3 J/cm(2)-Sham; n=8), LLLT at a dose of 21 J/cm(2) sham rats (21 J/cm(2)-Sham; n=8), placebo HF rats (P-HF; n=9), LLLT at a dose of 3 J/cm(2) HF rats (3 J/cm(2)-HF; n=8), and LLLT at a dose of 21 J/cm(2) HF rats (21 J/cm(2)-HF; n=8). Four weeks after myocardial infarction or sham surgery, rats were subjected to LLLT (InGaAlP 660 nm, spot size 0.035 cm(2), output power 20 mW, power density 0.571 W/cm(2), energy density 3 or 21 J/cm(2), exposure time 5.25 s and 36.75 s) on the right gastrocnemius for 10 consecutive days. LLLT reduced plasma IL-6 levels (61.3 %; P<0.01), TNF-/IL-10 (61.0 %; P<0.01) and IL-6/IL-10 ratios (77.3 %; P?<?0.001) and increased IL-10 levels (103 %; P<0.05) in the 21 J/cm(2)-HF group. Moreover, LLLT reduced the TNF-(20.1 % and 21.3 %; both P=<0.05) and IL-6 levels (54.3 % and 37.8 %; P?<0.01 and P<0.05, respectively) and the IL-6/IL-10 ratio (59.7 % and 42.2 %; P<?0.001 and P<0.05, respectively) and increased IL-10 levels (81.0 % and 85.1 %; both P<0.05) and the IL-10/TNF- ratio (171.5 % and 119.8 %; P<0.001 and P<0.05, respectively) in the gastrocnemius in the 3 J/cm(2)-HF and 21 J/cm(2)-HF groups. LLLT showed systemic and skeletal muscle anti-inflammatory effects in rats with HF.

Photomed Laser Surg.  2011 Feb 24. [Epub ahead of print]

Low-Level Laser Irradiation Alters Cardiac Cytokine Expression Following Acute Myocardial Infarction: A Potential Mechanism for Laser Therapy.

Yang Z, Wu Y, Zhang H, Jin P, Wang W, Hou J, Wei Y, Hu S.

1 Department of Surgery, Cardiovascular Institute and Fu Wai Hospital , Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China .


Abstract Objectives: Low-level laser irradiation (LLLI) has the potential of exerting cardioprotective effect following myocardial infarction (MI). The authors hypothesized that LLLI could influence the expression of cardiac cytokines and contribute to the reversal of ventricular remodeling. Background: LLLI regulates the expression of cytokines after tissue damage. However, little is known concerning the alteration of the cardiac cytokine expression profile after LLLI.

Methods: MI was created by coronary ligation. The surviving rats were divided randomly into laser and control groups. 33 rats were exposed to a diode laser (635?nm, 5?mW, CW, laser, beam spot size 0.8?cm(2), 6?mW/cm(2), 150?sec, 0.8?J, 1J/cm(2)) as laser group. Another 33 rats received only coronary ligation and served as control group. 28 rats received a thoracotomy without coronary ligation (sham group). One day after laser irradiation, 5 rats from each group were sacrificed and the heart tissues were analyzed by cytokine antibody arrays. Enzyme-linked immunosorbent assay (ELISA) was performed to confirm its reliability. Two weeks after MI, cardiac function and structure were evaluated by echocardiography and histological study. Results: Cytokine antibody array indicated 4 cytokines were significantly changed after laser therapy. ELISA confirmed that granulocyte-macrophage colony stimulating factor and fractalkine were the cytokines involved in the response to therapeutic laser irradiation. However, there was no difference in cytokine release between various groups at 2 weeks after MI. Although LLLI did not improve the damaged heart function, it did reduce the infarct area expansion.

Conclusions: The antibody-based protein array technology was applied for screening the cytokine expression profile following MI, with or without laser irradiation. The expression of multiple cytokines was regulated in the acute phase after LLLI. Our results revealed a potential novel mechanism for applying laser therapy to the treatment of heart disease.

Vopr Kurortol Fizioter Lech Fiz Kult.  2010 Nov-Dec;(6):12-5.

Potentials of combined non-medication therapy of arterial hypertension associated with ischemic heart disease.

[Article in Russian]

Achilov AA, Lebedeva OD, Bulatetskaia LS, Usmonzoda DU, Belov AS, Kotov SA, Achilova ShA, Rykov SV.


The objective of the present work was to develop methods and a scientifically sound rationale for the optimization of clinical results of the treatment of patients presenting with arterial hypertension (AH) associated with coronary heart disease (CHD) using red and infra-red (IR) low-intensity laser irradiation. The study included a total of 90 patients of working age (62.2% of men and 37.8% of women) having AH associated with coronary heart disease and abnormal blood lipid spectrum. They were allocated to three groups each comprising 30 patients matched for age, sex, severity of clinical conditions, and character of maintenance medicamental therapy. Patients of group 1 were treated by intravenous blood irradiation with laser light; those in group 2 were given similar treatment in combination with external low-intensity IR laser therapy; patients of group 3 received basal medicamental therapy. The study allowed the clinical value of different variants of low-intensity laser therapy to be assessed as regards efficiency of correction of hypersympaticotony, disturbances of lipid metabolism, and refractoriness to pharmaceutical products as well as improved tolerance of physical exercises, anti-hypertensive, anti-ischemic, and anti-anginal effects, diastolic function, and quality of life as targets of the comprehensive rehabilitative program designed for patients with AH associated with coronary heart disease. An optimized therapeutic modality is proposed for patients presenting with these pathologies based on the application of the low-intensity laser irradiation regime characterized by mutual amplification and complementary action of red and infrared lights.

J Cell Mol Med. 2009 Sep 1. [Epub ahead of print]

Low Level Laser Irradiation Precondition to Create Friendly Milieu of Infarcted Myocardium and Enhance Early Survival of Transplanted Bone Marrow Cells.


Zhang H, Hou JF, Shen Y, Wang W, Wei YJ, Hu S.

Department of Surgery, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

We hypothesized that low-level laser irradiation (LLLI) precondition prior to cell transplantation might remodel the hostile milieu of infarcted myocardium and subsequently enhance early survival and therapeutic potential of implanted bone-marrow mesenchymal stem cells (BMSCs). Therefore, in this study we wanted to address: (1) whether LLLI pretreatment change the local cardiac micro-environment after MI; and (2) whether the LLLI preconditions enhance early cell survival and thus improve therapeutic angiogenesis and heart function. Myocardial infarction was induced by left anterior descending artery ligation in female rats. A 635 nm, 5 mW diode laser was performed with energy density of 0.96 J/cm(2) for 150 seconds for the purpose of myocardial precondition. Three weeks later, qualified rats were randomly received with LLLI precondition (n=26) or without LLLI precondition (n=27) for LLLI precondition study. Rats received thoracotomy without coronary ligation were served as sham group (n=24). For the following cell survival study, rats were randomly received serum-free culture media injection (n=8), LLLI precondition and culture media injection (n=8), 2 millions male BMSCs transplantation without LLLI pretreatment (n=26) and 2 millions male BMSCs transplantation with LLLI precondition (n=25). Vascular endothelial growth factor (VEGF), glucose-regulated protein 78 (GRP78), superoxide dismutase (SOD) and malondialdehyde (MDA) in the infarcted myocardium were evaluated by Western blotting, real-time polymerase chain reaction (real-time PCR) and colorimetry, respectively, at 1 hour, 1 day and 1 week after laser irradiation. Cell survival was assayed with quantitative real-time PCR to identify Y chromosome gene and apoptosis was assayed with TUNEL staining. Capillary density, myogenic differentiation and left ventricular function were tested by immunohistochemistry and echocardiography, respectively, at 1 week. After LLLI precondition, increased VEGF and GRP78 expression, as well as the enhanced SOD activity and inhibited MDA production, was observed. Compared with BMSCs transplantation and culture media injection group, although there was no difference in the improved heart function and myogenic differentiation, LLLI precondition significantly enhanced early cell survival rate by 2-fold, decreased the apoptotic percentage of implanted BMSCs in infarcted myocardium and thus increased the number of newly formed capillaries. Taking together, LLLI precondition could be a novel non-invasive approach for intraoperative cell transplantation to enhance cell early survival and therapeutic potential.

Cardiovasc Res. 2009 Jun 8. [Epub ahead of print]

Low-level laser irradiation inhibits abdominal aortic aneurysm progression in apolipoprotein E-deficient mice.

Gavish L, Rubinstein C, Bulut A, Berlatzky Y, Beeri R, Gilon D, Gavish L, Harlev M, Reissman P, Gertz SD.

Department of Anatomy and Cell Biology, The Hebrew University-Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel.

AIMS: Increased early detection of abdominal aortic aneurysm (AAA) and the severe complications of its current treatment have emphasized the need for alternative therapeutic strategies that target pathogenetic mechanisms of progression and rupture. Recent in vitro studies from our laboratory have shown that low-level laser irradiation (LLLI) (780 nm) modifies cellular processes fundamental to aneurysm progression. The present study was designed to determine whether LLLI retards the progression of suprarenal AAA in vivo. METHODS AND RESULTS: High-frequency ultrasonography (0.01 mm resolution) was used to quantify the effect of LLLI on aneurysmatic aortic dilatation from baseline to 4 weeks after subcutaneous infusion of angiotensin II by osmotic minipumps in the apolipoprotein E-deficient mouse. At 4 weeks, seven of 15 non-irradiated, but none of the 13 LLLI, mice had aneurysmal dilatation in the suprarenal aneurysm-prone segments that had progressed to >/=50% increase in maximal cross-sectional diameter (CSD) over baseline (P = 0.005 by Fisher’s exact test). The mean CSD of the suprarenal segments (normalized individually to inter-renal control segments) was also significantly lower in irradiated animals (LLLI vs. non-irradiated: 1.32 +/- 0.14 vs. 1.82 +/- 0.39, P = 0.0002 by unpaired, two-tailed t-test) with a 94% reduction in CSD at 4 weeks compared with baseline. M-mode ultrasound data showed that reduced radial wall velocity seen in non-treated was significantly attenuated in the LLLI mice, suggesting a substantial effect on arterial wall elasticity. CONCLUSION: These in vivo studies, together with previous in vitro studies from this laboratory, appear to provide strong evidence in support of a role for LLLI in the attenuation of aneurysm progression. Further studies in large animals would appear to be the next step towards testing the applicability of this technology to the human interventional setting.

International Symposium for Modernization of TCM, Graz, Austria: 16, May 2009

Laser therapy and cost-effective cardiovascular care


Rindge, D.  Center for Cooperative Medicine /  Healing Light Seminars, Melbourne, Florida USA


Cardiovascular disease is the leading cause of death throughout the world, accounting for 42% of mortality and €192 billion in the European Union annually. Current conventional treatments have been limited almost exclusively to surgery, angioplasty and pharmaceutical drugs.  These are often expensive and carry risk, and they may do little to address the underlying disease process.

Atherosclerosis is the well-accepted, root cause of virtually all cardiovascular disease.  It occurs within arteries in nearly all humans. Any method to reverse or prevent this progressive, degenerative process promises to raise cardiovascular care to a new level, increasing longevity and quality of life.  Laser therapy has been documented to restore normal endothelial function, inhibiting or even reversing atherosclerosis, and it has been given high marks in research across a wide range of cardiovascular conditions.

This presentation will explore the documented benefits of laser therapy in angina, atherosclerosis, coronary artery disease, hypertension, hyperlipidemia, myocardial infarction and stroke as well as treatment methods and mechanisms.  Increased vital energy to cells, tissues and throughout the entire organism through stimulation of mitochondrial production of ATP may be the most profound effect of laser therapy, but it is not the only one.  Laser therapy also induces the secondary emission of biophotons by the blood itself and may also increase electrostatic charge within the circulating volume to hold colloids in suspension and prevent the aggregation of platelets and other formed elements, inhibiting or even reversing the formation of thrombi and plaque.

Photomed Laser Surg. 2009 Apr;27(2):227-33.

Implantation of low-level laser irradiated mesenchymal stem cells into the infarcted rat heart is associated with reduction in infarct size and enhanced angiogenesis.

Tuby H, Maltz L, Oron U.

Department of Zoology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel.

OBJECTIVE: The aim of the present study was to evaluate the possible beneficial effects of implantation of laser-irradiated mesenchymal stem cells (MSCs) into the infarcted rat heart. BACKGROUND DATA: It was demonstrated that low-level laser therapy (LLLT) upregulates cytoprotective factors in ischemic tissues.MATERIALS AND METHODS: MSCs were isolated from rat bone marrow and grown in culture. The cells were laser irradiated with a Ga-Al-As laser (810 nm wavelength), labeled with 5-bromo-2’deoxyuridine (BrdU), and then implanted into infarcted rat hearts. Non-irradiated cells were similarly labeled and acted as controls. Hearts were excised 3 wk later and cells were stained for BrdU and c-kit immunoreactivity. RESULTS: Infarcted hearts that were implanted with laser-treated cells showed a significant reduction of 53% in infarct size compared to hearts that were implanted with non-laser-treated cells. The hearts implanted with laser-treated cells prior to implantation demonstrated a 5- and 6.3-fold significant increase in cell density that positively immunoreacted to BrdU and c-kit, respectively, as compared to hearts implanted with non-laser-treated cells. A significantly 1.4- and 2-fold higher level of angiogenesis and vascular endothelial growth factor, respectively, were observed in infarcted hearts that were implanted with laser-treated cells compared to non-laser-treated implanted cells. CONCLUSION: The findings of the present study provide the first evidence that LLLT can significantly increase survival and/or proliferation of MSCs post-implantation into the ischemic/infarcted heart, followed by a marked reduction of scarring and enhanced angiogenesis. The mechanisms associated with this phenomenon remain to be elucidated in further studies.

Klin Lab Diagn. 2009 Apr;(4):19-21.

Platelet aggregatory properties in patients with chronic pancreatitis and possibilities of correcting their impairments.

[Article in Russian]

Burduli NM, Gutnova SK.

The purpose of the investigation was to study the impact of low-intensity laser therapy (LILT) on platelet aggregatory properties in patients with chronic pancreatitis (CP) on an exacerbation. A total of 105 patients aged 36 to 77 years who were divided into a study group (n = 60) and a control one (n = 45) were examined. Thirty persons who formed a healthy group were additionally examined. In the study group patients, drug therapy was supplemented by LILT via various methods. The control group received only drug therapy. The investigation revealed that patients with CP on an exacerbation showed diverse changes in platelet aggregatory properties towards hyperaggregation. LILT was ascertained to have a normalizing effect on platelet aggregatory properties in the study group patients.

Rev Bras Cir Cardiovasc. 2009 Mar;24(1):88-91.

Low level laser therapy in acute dehiscence saphenectomy: therapeutic proposal.

[Article in English, Portuguese]

Pinto NC, Pereira MH, Stolf NA, Chavantes MC.

Service of Laser Medical Center, Heart Institute – InCor/HC – Faculty of Medicine of the Universitof São Paulo, São Paulo, Brasil.

Dehiscence is a feared complication after major surgeries. Patient who had undergone coronary artery bypass grafting developed saphenectomy’s dehiscence on lower limb with edema and pain on the 15th postoperative day. Conventional treatment had been initially performed without clinical improvement. On the 30th postoperative day only Low Level Laser Therapy (LLLT) was applied punctually around surgical wounds edge. The results revealed granulated tissue, reduction of inflammatory process and analgesic effect since the first application. In this pilot study, LLLT has shown a considerable role as a wound healing agent, through a new proposal for efficient, safe and noninvasive therapy.

Proc SPIE, Vol 7161, 7162S (2009); doi: 10.1117/12.808050 February 2009

Laser therapy in cardiovascular disease

Rindge, D. Center for Cooperative Medicine / Healing Light Seminars, Melbourne, Florida, USA

Cardiovascular disease is the number one cause of death worldwide.  It is broadly defined to include anything which adversely affects the heart or blood vessels.  One-third of Americans have one or more forms of it.  By one estimate, average human life expectancy would increase by seven years if it were eliminated.

The mainstream medical model seeks mostly to “manage” cardiovascular disease with pharmaceuticals or to surgically bypass or reopen blocked vessels via angioplasty. These methods have proven highly useful and saved countless lives.  Yet drug therapy may be costly and ongoing, and it carries the risk of side effects while often doing little or nothing to improve underlying health concerns.  Similarly, angioplasty or surgery are invasive methods which entail risk.

Laser therapy regenerates tissue, stimulates biological function, reduces inflammation and alleviates pain.  Its efficacy and safety have been increasingly well documented in cardiovascular disease of many kinds.  In this article we will explore the effects of laser therapy in angina, atherosclerosis, coronary artery disease, hypertension, hyperlipidemia, myocardial infarction, stroke and other conditions.  The clinical application of various methods of laser therapy, including laserpuncture and transcutaneous, supravascular and intravenous irradiation of blood will be discussed. Implementing laser therapy in the treatment of cardiovascular disease offers the possibility of increasing the health and wellbeing of patients while reducing costs and enhancing safety of medical care.  ©2009 COPYRIGHT SPIE – The International Society for Optical Engineering.  Downloading of this abstract is for personal use only.

Mol Cell Cardiol. 2009 Jan;46(1):4-14. Epub 2008 Sep 30.

Near infrared light protects cardiomyocytes from hypoxia and reoxygenation injury by a nitric oxide dependent mechanism.

Zhang R, Mio Y, Pratt PF, Lohr N, Warltier DC, Whelan HT, Zhu D, Jacobs ER, Medhora M, Bienengraeber M.

Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53326, USA.

Photobiomodulation with near infrared light (NIR) provides cellular protection in various disease models. Previously, infrared light emitted by a low-energy laser has been shown to significantly improve recovery from ischemic injury of the canine heart. The goal of this investigation was to test the hypothesis that NIR (670 nm) from light emitting diodes produces cellular protection against hypoxia and reoxygenation-induced cardiomyocyte injury. Additionally, nitric oxide (NO) was investigated as a potential cellular mediator of NIR. Our results demonstrate that exposure to NIR at the time of reoxygenation protects neonatal rat cardiomyocytes and HL-1 cells from injury, as assessed by lactate dehydrogenase release and MTT assay. Similarly, indices of apoptosis, including caspase 3 activity, annexin binding and the release of cytochrome c from mitochondria into the cytosol, were decreased after NIR treatment. NIR increased NO in cardiomyocytes, and the protective effect of NIR was completely reversed by the NO scavengers carboxy-PTIO and oxyhemoglobin, but only partially blocked by the NO synthase (NOS) inhibitor L-NMMA. Mitochondrial metabolism, measured by ATP synthase activity, was increased by NIR, and NO-induced inhibition of oxygen consumption with substrates for complex I or complex IV was reversed by exposure to NIR. Taken together these data provide evidence for protection against hypoxia and reoxygenation injury in cardiomyocytes by NIR in a manner that is dependent upon NO derived from NOS and non-NOS sources.

Lasers Surg Med. 2008 Jul;40(5):371-8.

Irradiation with 780 nm diode laser attenuates inflammatory cytokines but upregulates nitric oxide in lipopolysaccharide-stimulated macrophages: implications for prevention of aneurysm progression.

Gavish L, Perez LS, Reissman P, Gertz SD.

Department of Anatomy and Cell Biology, The Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel.

BACKGROUND AND OBJECTIVES: Low level laser irradiation (LLLI) has been shown to reduce inflammation in a variety of clinical situations. We have shown that LLLI (780 nm) increases aortic smooth muscle cell proliferation and matrix protein secretion and modulates activity and expression of matrix metalloproteinases. Inflammation is a major component of arteriosclerotic diseases including aneurysm. Macrophage recruitment and secretion of pro-inflammatory cytokines and the vasodilator, nitric oxide (NO), are central to most immune responses in the arterial wall. The present study was designed to determine the effect of LLLI on cytokine gene expression and secretion as well as gene expression of inducible nitric oxide synthase (iNOS) and NO production in lipopolysaccharide (LPS)-stimulated macrophages. STUDY DESIGN/MATERIALS AND METHODS: Murine monocyte/macrophages (RAW 264.7) were irradiated with a 780 nm diode laser (2 mW/cm(2), 2.2 J/cm(2)) during stimulation with LPS (0, 0.1, and 1 microg/ml). Gene expression of chemokines, cytokines, and iNOS were assessed by RT-PCR. Secretion of interleukin (IL)-1beta and monocyte chemotactic protein (MCP)-1 and NO were assessed by ELISA and the Griess reaction, respectively. RESULTS: LLLI reduced gene expression of MCP-1, IL-1alpha, IL-10 (P<0.01), IL-1beta, and IL-6 (P<0.05) when cells were stimulated by 1 microg/ml LPS. LLLI reduced LPS-induced secretion of MCP-1 over non-irradiated cells by 17+/-5% and 13+/-5% at 12 hours (0.1 and 1 microg/ml LPS; P<0.01 and P=0.05, respectively), and reduced IL-1beta by 22+/-5% and 25+/-9% at 24 hours (0.1 and 1 microg/ml LPS, P=0.01 and P=0.06, respectively). However, LLLI increased NO secretion after 12 hours (LLLI vs. Control: without LPS, 1.72+/-0.37 vs. 0.95+/-0.4 microM, P<0.05; 0.1 microg/ml LPS, 7.46+/-1.62 vs. 4.44+/-1.73 microM, P=0.06; 1 microg/ml LPS, 10.91+/-3.53 vs. 6.88+/-1.52 microM, P<0.05). CONCLUSIONS: These properties of LLLI, with its effects on smooth muscle cells reported previously, may be of profound therapeutic relevance for arterial diseases such as aneurysm where inflammatory processes and weakening of the matrix structure of the arterial wall are major pathologic components.

Klin Med (Mosk). 2008;86(6):44-7.

Laser Doppler fluometry in assessment of endothelium state in patients with coronary heart disease and its correction by intravenouls laser irradiation of blood.

[Article in Russian]

Burduli NM, Gazdanova AA.

Influence of laser therapy on vascular endothelium function in patients with stable angina pectoris, detected by the method of laser Doppler fluorimetry, was studied. 77patients with stable angina pectoris were divided into 2 groups. In control group only medicamentous therapy was used, in main group a course of intravenous blood laser irradiation was carried out additionally. The increase of initially decreased mean index of microcirculation and index of microcirculation after acetylcholine iontophoresis was noticed. Tendency to increase of endothelial oscillations and capillary blood flow under influence of intravenous blood laser irradiation was noticed too. Laser therapy can be considered an effective method of increase of endothelial functional activity in patients with stable angina pectoris.

Kardiol Pol. 2007 Jan;65(1):13-21; discussion 22-3.

Laser biostimulation in end-stage multivessel coronary artery disease–a preliminary observational study.

[Article in English, Polish]

Zyci?ski P, Krzemi?ska-Paku?a M, Peszy?ski-Drews C, Kierus A, Trzos E, Rechci?ski T, Figiel L, Kurpesa M, Plewka M, Chrzanowski L, Drozdz J.

II Katedra i Klinika Kardiologii Uniwersytetu Medycznego, ul. Kniaziewicza 1/5, ?ód?.

BACKGROUND: Low-energy laser radiation through its direct influence on tissue repair processes without heating effect may have vital importance in the therapy of patients with advanced coronary artery disease (CAD). AIM: The introductory assessment of the effects of laser biostimulation applied to patients with advanced multivessel CAD. METHODS: 39 patients with advanced CAD were assigned (mean age 64.8+/-9.6, male gender 64%, CCS class 2.5+/-0.5, EF=46+/-11%, 69% with a history of acute myocardial infarction), to undergo two sessions of irradiation of low-energy laser light on skin in the chest area from helium-neon B1 lasers. The time of irradiation was 15 minutes while operations were performed 6 days a week for one month. Before including the patients in the experimental group a full clinical evaluation, basic biochemical tests, ECG, 24h Holter recordings, 6-minute walk test, treadmill test using Bruce protocol and full echocardiographic examination were performed. After the first and second period of laser therapy with a one-month break between them analogical parameters with the initial examination were measured. RESULTS: No side effects associated with the laser biostimulation or performed clinical tests were noted. Lower CCS class (2.5+/-0.5 –> 2.2+/-0.4 –> 2.0+/-0.4, p<0.001), higher exercise capacity (5.1+/-2.2 –> 5.8+/-2.2 –> 6.6+/-2.5 [METS], p=0.023), longer exercise time (257+/-126 –> 286+/-127 –> 325+/-156 [s], p=0.06), less frequent angina symptoms during the treadmill test (65% –> 44% –> 38%, p=0.02), longer distance of 6-minute walk test (341+/-93 –> 405+/-113 –> 450+/-109 [m], p <0.001), lower systolic blood pressure values (SP 130+/-14 –> 125+/-12 –> 124+/-14 [mmHg], p=0.05) and trend towards less frequent 1 mm ST depression lasting 1 min during Holter recordings were noted. CONCLUSIONS: An improvement of functional capacity and less frequent angina symptoms during exercise tests without a significant change in the left ventricular function were observed. Laser biostimulation in short-term observation was a very safe method. These encouraging results should be confirmed in a larger, placebo-controlled study.

Coron Artery Dis. 2006 May;17(4):385-90.

Long-term results following percutaneous myocardial laser therapy.

Salem M, Rotevatn S, Nordrehaug JE.

Department of Heart Disease, Haukeland University Hospital, Bergen, Norway.

OBJECTIVES: The usefulness and safety of percutaneous myocardial laser therapy in selected patients have been identified in previous 1-year randomized trial reports, including that from a double-blind, sham-controlled trial we independently conducted. We aimed to determine whether the 1-year effects are maintained through a long-term, longitudinal follow-up. METHODS: Patients (n=77) with chronic, stable, medically refractory angina (class III or IV) not amenable to conventional revascularization and with evidence of reversible ischemia, ejection fraction > or =25%, and myocardial wall thickness > or =8 mm were treated with percutaneous myocardial laser. After the 1-year follow-up and disclosure of all randomized assignments as prespecified in the respective study protocol, patients were followed up longitudinally for a mean of 3 years for angina class, left ventricular ejection fraction, medication usage, and adverse events. RESULTS: No procedural mortality, myocardial infarction, or cerebral embolism occurred. Pericardiocentesis was required in two patients (2.6%). Cardiac event-free survival was 88% at 1 year and 66% at late follow-up. Mean Canadian Cardiovascular Society angina class was significantly improved from baseline (3.2+/-0.4) at 1 year (2.2+/-1.1, P<0.001) and at a mean of 3 years (1.9+/-1.2, P<0.001). Nitrate usage was significantly reduced at late follow-up; however, ejection fraction did not change over time. In a multivariate analysis, angina improvement at 1 year was found to be a significant independent predictor of both survival and angina improvement at late follow-up. CONCLUSION: We conclude that percutaneous myocardial laser therapy in selected patients with severe, medically refractory angina not treatable with conventional revascularization induces significant and sustained symptomatic benefit.

EMLA Laser Health J 2007;2:46-67
10th International Congress of the European Medical Laser Association (EMLA) Laser Prague 2005


Successful treatment of finger atheroembolism with low-level laser irradiation (LLLI): report of a case.

Kazemikhou N. 1, Mokmeli S. 1, Gousheh S.J. 2, Mir-Abdul-Hagh M. 3
1. Laser clinic, Milad hospital, Tehran, Iran.
2. Division of Reconstructive and Microsurgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3. Bagiyatollah hospital, Molla-Sadra St., Bagiyatollah University of Medical Sciences, Tehran

Atheroembolism constitutes a subset of acute arterial occlusion, in which, multiple small deposits of fibrin, platelet, and cholesterol debris embolize from proximal atherosclerotic lesions or aneurysmal sites. Ischemia resulting from atheroemboli in extremities is notoriously difficult to treat and circulatory disturbance in these regions usually results in amputation. During past 30 years, low-level lasers have been widely used in medical fields. Low-level laser irradiation in red and near infrared region, locally or intravenously may result in vasodilatation, increased tissue perfusion and neovascularization.

A 64 years-old Iranian man referred to our clinic with acute atheroembolism of right index finger distal phalanx. There was no predisposing cardiovascular factor except for a mild hypercholesterolemia, and physical examination was generally normal. During 12 sessions (about 30 days) of low-level laser therapy with infrared light, 980 nm/ 100 mW and red light, 650 nm/ 30 mW, associated with 3 sessions of intravenous red light laser, 2 mW, ischemia disappeared and the finger was quite normal. On 12 months of follow-up, the patient was uneventful.

Our observations during treatment process confirmed the results of previous experimental and clinical observations about LLLI effects on tissue healing of ischemic areas demonstrating that delivery of laser energy to the ischemia-induced necrotic area may have an important beneficial effect on patients with acute thromboembolism of fingers, preventing them from amputation or other invasive surgical interventions. However, additional studies and more clinical evidences are to be needed to demonstrate the various aspects of this application of lasers in medical practice.

Photomed Laser Surg. 2006 Apr;24(2):111-20.

Photoengineering of tissue repair in skeletal and cardiac muscles.

Oron U.

Department of Zoology, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel.

This review discusses the application of He-Ne laser irradiation to injured muscles at optimal power densities and optimal timing, which was found to significantly enhance (twofold) muscle regeneration in rats and, even more, in the cold-blooded toads. Multiple and frequent (daily) application of the laser in the toad model was found to be less effective than irradiation on alternate days. It was found that in the ischemia/reperfusion type of injury in the skeletal leg muscles (3 h of ischemia), infrared Ga-Al-As laser irradiation reduced muscle degeneration, increased the cytoprotective heat shock proteins (HSP-70i) content, and produced a twofold increase in total antioxidants. In vitro studies on myogenic satellite cells (SC) revealed that phototherapy restored their proliferation. Phototherapy induced mitogen-activated protein kinase/extracellular signal-regulated protein kinase (MAPK/ERK) phosphorylation in these cells, probably by specific receptor phosphorylation. Cell cycle entry and the accumulation of satellite cells around isolated single myofibers cultured in vitro was also stimulated by phototherapy. Phototherapy also had beneficial effects on mouse, rat, dog and pig ischemic heart models. In these models, it was found that phototherapy markedly and significantly reduced (50-70%) the scar tissue formed after induction of myocardial infarction (MI). The phototherapeutic effect was associated with reduction of ventricular dilatation, preservation of mitochondria and elevation of HSP- 70i and ATP in the infarcted zone. It is concluded that phototherapy using the correct parameters and timing has a markedly beneficial effect on repair processes after injury or ischemia in skeletal and heart muscles. This phenomenon may have clinical applications.

Progress in Low-level Laser Therapy


Jackson Streeter
PhotoThera, Carlsbad, CA;

This paper appears in: Lasers and Electro-Optics Society, 2006. LEOS 2006. 19th Annual Meeting of the IEEE
Publication Date: Oct. 2006
On page(s): 665-666
ISBN: 0-7803-9555-7
INSPEC Accession Number: 9364378
Digital Object Identifier: 10.1109/LEOS.2006.278888
Current Version Published: 2007-01-15

The presentation covers fundamental operating principles of some of the most widely used methods of low-level laser therapy (LLLT). It includes also recently developed LLLT technologies and medical devices such as LLLT cardiovascular and brain therapy, tissue regeneration and pain relive. The mechanism of LLLT involving interaction with mitochondria. The effects of LLLT are wavelength specific upon a known mitochondrial receptor (cytochrome C oxidase). Targeting of this receptor results in formation of adenosine triphosphate (ATP), enhanced mitochondrial survival and maintenance of cytochrome C oxidase activity

Likars’ka sprava. 2001; (5-6): 111-114.


Evaluation of quantum therapy for the treatment of stable angina

[Otsinka efektyvnosti vplyvu kvantovoi terapii na perebig stabil’noi stenokardii].

A total of 68 patients were examined. Of these, 21 had functional class (FC) I stabile angina, 23 presented with FC II angina, 24 had FC III angina. Instituted in the control group patients (n = 30) was standard antianginal therapy (SAT). Laser therapy against the background of SAT employed has been found to improve the functional state of the myocardium, enhance tolerance to physical loads, improve indices for intracardiac hemodynamics. HeNe laser irradiation has an analgecizing effect. Patients with FC I-III exertional angina can derive benefit from laser therapy due to its cardioprotective effect. Positive hemodynamic shifts were accompanied by improvement in general health of patients manifested by lower frequency of angina attacks and episodes of pain-free ischemia of the myocardium. Laser therapy had an effect on relation between painful and painless ischemia of the myocardium as evidenced by a predominant decrease in pain-free episodes of myocardial ischemia, this being regarded as a prognostically favourable fact.

J Clin Laser Med Surg. 2003 Aug;21(4):231-5.

A preliminary investigation into light-modulated replication of nanobacteria and heart disease.

Sommer AP, Oron U, Pretorius AM, McKay DS, Ciftcioglu N, Mester AR, Kajander EO, Whelan HT.

Central Institute of Biomedical Engineering, University of Ulm, 89081 Ulm, Germany.

OBJECTIVE: The purpose of this preliminary study is to evaluate the effect of various wavelengths of light on nanobacteria (NB). BACKGROUND DATA: NB and mitochondria use light for biological processes. NB have been described as multifunctional primordial nanovesicles with the potential to utilize solar energy for replication. NB produce slime, a process common to living bacteria. Slime release is an evolutionary important stress-dependent phenomenon increasing the survival chance of individual bacteria in a colony. In the cardiovascular system, stress-induced bacterial colony formation may lead to a deposition of plaque. METHODS: Cultured NB were irradiated with NASA-LEDs at different wavelengths of light: 670, 728 and 880 nm. Light intensities were about 500k Wm(-2), and energy density was 1 x 10(4) J m(-2). RESULTS: Monochromatic light clearly affected replication of NB. Maximum replication was achieved at 670 nm. CONCLUSIONS: The results indicate that suitable wavelengths of light could be instrumental in elevating the vitality level of NB, preventing the production of NB-mediated slime, and simultaneously increasing the vitality level of mitochondria. The finding could stimulate the design of cooperative therapy concepts that could reduce death caused by myocardial infarcts.

Ter Arkh. 2003;75(12):19-23.

Effect of membrane-stabilizing magnetolaser therapy on cardiodynamics in patients with ischemic heart disease

[Article in Russian]

Vasil’ev AP, Senatorov IuN, Strel’tsova NN, Malishevski? MV, Dubova TV, Zykova EL.

AIM: To evaluate cardiodynamic changes in response to magnetolaser therapy (MLT) and these changes links with lipid shifts in cell membrane. MATERIAL AND METHODS: The study enrolled 50 patients with effort angina (functional class II-III). Of them, 37 patients were exposed to 10-day courses of MLT, 13 patients were exposed to sham procedures. Before the treatment and 3 months after it measurements were made of lipid peroxidation (LPO) products, structure of erythrocytic membrane and cardiodynamic parameters. RESULTS: MLT resulted in a significant reduction of LPO products, stabilization of cell membrane structure and positive shifts in cardiodynamics. Correlation was found between the above parameters. CONCLUSION: Improvement of inotropic, diastolic functions of the myocardium and abatement of cardiac remodeling in coronary heart disease patients in response to MLT is realized primarily due to structural stabilization of cell membrane lipid biolayer.

Klin Med (Mosk). 2003;81(1):24-7.


Clinico-functional efficacy of medicinal and photon stabilization of cell membrane in patients with angina pectoris

[Article in Russian]

Vasil’ev AP, Senatorov IuN, Strel’tsova NN, Gorbunova TIu.

Modification of erythrocytic membrane and the trend in clinicofunctional indices were studied in 90 patients with angina of effort (FC I-IV) in the course of treatment with a combination of membranoprotective drugs (group 1), magneto-laser radiation (group 2) and imitation of laser radiation (group 3). In patients of groups 1 and 2 the treatment resulted in stabilization of cell membrane accompanied with a hypotensive effect and increased exercise tolerance due to more effective cardiac performance.

Chin Med J (Engl). 2004 Oct;117(10):1476-80

Low-power Helium-Neon laser irradiation enhances the expression of VEGF in murine myocardium.

Zhang WG, Wu CY, Pan WX, Tian L, Xia JL.

Department of Anatomy and Histoembryology, Peking University Health Science Center, Beijing 100083, China.

BACKGROUND: Low-power helium-neon (He-Ne) lasers have been increasingly widely applied in the treatment of cardiovascular diseases, and its vasodilation effect has been proven. The aim of this study was to determine the effects of low-power He-Ne laser irradiation directed at the precardial region of Wistar rats on capillary permeability in the myocardium and the expression of myocardial vascular endothelial growth factor (VEGF). METHODS: Sixteen rats were divided randomly into control and irradiated groups (n = 8, each). A He-Ne laser (632.8 nm) was applied to the irradiated group with a dose of 60.5 J/cm(2). Ferritin was perfused into the left femoral vein and capillary permeability was examined under an electron microscope. VEGF expression in the myocardium was investigated by immunohistochemical methods, RT-PCR, and image analysis. RESULTS: The ultrastructures of the myocardial capillaries were examined. Compared to the control group, more high-density granules (ferritin), which were present within the capillary endothelium and the mitochondrions of myocardial cells in the internal layer of the myocardium, were observed in the irradiated group. VEGF staining of the myocardium was stronger in the irradiated group than that in the control group. The optic density of the irradiated group (0.246 +/- 0.015) was significantly higher than that of the control group (0.218 +/- 0.012, P < 0.05). Finally, the levels of RT-PCR products of VEGF165 mRNA were 2.79 times higher in irradiated rats than in the control rats. CONCLUSIONS: Our study demonstrates that He-Ne laser irradiation (in doses of 60.5 J/cm(2)) increases myocardial capillary permeability and the production of VEGF in myocardial microvessels and in myocardium. Our study provides experimental morphological evidence that myocardial microcirculation can be improved using He-Ne laser irradiation.

Proc. SPIE Vol. 2100, p. 284-291, Cell and Biotissue Optics: Applications in Laser Diagnostics and Therapy

Fifteen-year experience with low-energy laser applications for patient treatment in emergency-care clinics

Nemtsev, Igor Z.; Lapshin, V. P.Valery, V. Tuchin; Ed.

Low energy lasers have been successfully applied for the treatment of more than 10 thousand patients in the Sklifosovsky Scientific Research Institute for Emergency Medicine. We have reviewed and generalized the results of clinical investigations of 34 patients with trauma of motor-locomotive system, 15 patients with muscular atrophy occurred as a result of lower extremity fracture immobilization, 124 patients with burns and of 88 post-infarction patients with ischemic heart disease, who underwent laser therapy with He-Ne laser, N2 Ultra-Violet and GaAs Infra-Red lasers.


S. Belousov, E. Galperin, E. Smetova

The purpose of this study was the evaluation of arterial relaxation ability in 15 patients with CAD before and after the course of infrared laser therapy. 18 controls (healthy men). With high-resolution ultrasound and impulse wave doppler we measured the increasing of volumic velocity flow (%) of the arteria poplitea at rest and during reactive hiperaemia (with increased flow causing endothelium-dependent dilatation). In controls flow-mediated mean dilatation was normal. Endothelial dysfunction is present in majority of patients with CAD, flow-mediated dilatation was much reduced of absent. Course of infrared laser therapy was benefit to restore endothelial function in patients with CAD.

Vopr Kurortol Fizioter Lech Fiz Kult. 2003 Jul-Aug;(4):10-3.


Efficacy of laser therapy in patients with ischemic heart disease

[Article in Russian]

Vasil’ev AP, Strel’tsova NN, Senatorov IuN.

Modification of erythrocytic membrane and the trend in clinicofunctional indices were studied in 93 patients with angina of effort (FC i-IV) in the course of treatment with laser radiation (group 1) and imitation of laser radiation (group 2). In patients of group 1 the treatment resulted in stabilization of cell membrane accompanied with positive cardiodynamic changes.

Klin Med (Mosk). 2002;80(4):31-3.


Diagnostic implications of changed red cell count with low-intensity laser radiation of blood in elderly patients with coronary heart disease

[Article in Russian]

Simonenko VB, Siuch NI, Vokuev IA.

Intravenous laser therapy in combination with medication was conducted in 41 elderly patients with coronary heart disease (633 nm, 1 mW, 124 mW/cm2). The study of qualitative and quantitative (osmotic resistance) erythrocyte indices of blood demonstrated the change of erythrocyte number in circulating blood by the third laser procedure. Frequency of these changes correlated with duration of the treatment course. Intravenous laser therapy had a wider spectrum of effects on erythrocyte number than medication. Changes in erythrocyte number in the peripheral blood upon intravenous laser radiation reflects efficiency of treatment of coronary heart disease patients.

Vopr Kurortol Fizioter Lech Fiz Kult. 2002 Jul-Aug;(4):9-11.

[Use of infrared laser therapy in patients with ischemic heart disease associated with diabetes mellitus type 2 in health resort]

[Article in Russian]

Zin’kovskaia TM, Zavrazhnykh LA, Golubev AD.

Infrared laser therapy (300 Hz) combined with balneotherapy and patients’ education is more effective than standard sanatorium rehabilitation in patients with ischemic heart disease associated with diabetes mellitus type 2. 81.8% patients showed good response manifesting in less frequent anginal attacks, episodes of pain and painless ischemia and lower doses of antianginal drugs. Systolic and diastolic arterial pressure lowered by 18 and 10 mm Hg on the average, respectively. Multimodality rehabilitation of IHD patients with type 2 diabetes mellitus improves hemostasis, carbohydrate and lipid metabolism. Coronary circulation response lasted for 24 weeks

Vopr Kurortol Fizioter Lech Fiz Kult. 2001 Jul-Aug;(4):3-6.

The efficiency of low-intensity laser radiation in the treatment of arterial hypertension complicated by ischemic heart disease

[Article in Russian]

Shuvalova IN, Klimenko IT, Svinina NG, Tsereteli MV, Zankina VG, Miasoed FR.

The efficiency of low-intensity laser radiation (LILR) was studied in the treatment of 291 patients with arterial hypertension and ischemic heart disease. Clinical grounds are given for use of LILR red and infrared rays in rehabilitation of hypertensive patients with ischemia. The rehabilitation regimens can be differentiated according to the disease severity, type of hemodynamics, state of cerebral circulation.

Vopr Kurortol Fizioter Lech Fiz Kult. 2001 Nov-Dec;(6):10-3.


Laser irradiation in the treatment of ischemic heart disease

[Article in Russian]

Vasil’ev AP, Strel’tsova NN, Senatorov IuN.

Cardiodynamic changes due to beta-blocker carvedilol and low-intensity infrared laser radiation were compared in 115 patients with ischemic heart disease (IHD). The comparison has shown a similar positive effect on heart contractility and diastolic function. This gave arguments for feasibility of laser beam usage as a neurohormonal modulator in IHD patients to reduce cardiac remodulation and prevent cardiac failure.

Vopr Kurortol Fizioter Lech Fiz Kult. 2003 May-Jun;(3):22-5.


Antioxidant action and therapeutic efficacy of laser irradiation of blood in patients with ischemic heart disease

[Article in Russian]

Volotovskaia AV, Ulashchik VS, Filipovich VN.

Laser irradiation in therapeutic doses (gamma = 632.8 nm, 14 mW) has an antioxidant effect in blood irradiation in vitro as shown by activation of superoxide dismutase (SOD) which is a key enzyme of the antioxidant system (AOS) and suppression of lipid peroxidation. Adjuvant supravascular He-Ne laser irradiation of blood in combined therapy of 82 patients with ischemic heart disease (IHD) produces a positive trend in the clinical picture, hemostasis, lipid metabolism, blood SOD activity. Thereby, this method of laser hemotherapy is recommended for use in IHD patients. The dependence of the treatment results on the initial blood AOS necessitates consideration of AOS state in deciding on laser therapy in this group of patients.

Vopr Kurortol Fizioter Lech Fiz Kult. 2002 Jul-Aug;(4):9-11.


Use of infrared laser therapy in patients with ischemic heart disease associated with diabetes mellitus type 2 in health resort

[Article in Russian]

Zin’kovskaia TM, Zavrazhnykh LA, Golubev AD.

Infrared laser therapy (300 Hz) combined with balneotherapy and patients’ education is more effective than standard sanatorium rehabilitation in patients with ischemic heart disease associated with diabetes mellitus type 2. 81.8% patients showed good response manifesting in less frequent anginal attacks, episodes of pain and painless ischemia and lower doses of antianginal drugs. Systolic and diastolic arterial pressure lowered by 18 and 10 mm Hg on the average, respectively. Multimodality rehabilitation of IHD patients with type 2 diabetes mellitus improves hemostasis, carbohydrate and lipid metabolism. Coronary circulation response lasted for 24 weeks.


V.A. Lyusov, K.G. Khutchumova, Y.N. Fedullaev

The Russian Medical State University, Moscow, Russia

The aim of the present investigation is to study the effect of low level laser on extrasystoles. The amount of citrasystoles is registered before treatment and on the 10th day after the therapy. 64 patients with angina pectoris underwent laser therapy. We registered patients with more than 200 supraventricular extrasystoles and with more than 100 ventricular extrasystoles before our therapy. The treatment was carried out the keep of GaAs laser – “Elat” (890 nm) with pulse regime. The region of projection of carotide sinuses and heart were irradiated with pulse repetition rate 150 – 300 Hz with expositions 20 min. The amount of extrasystoles was counted by Holter monitor device. Laser therapy lessened supraventricular citrasystoles from 308,4±29,6 to 134,6±21,8 (p<0,001) and ventricular extrasystoles – from 182,1±31,3 to 41,5±16,3 (p<0,05) in patients with 1-3 functional class. We observed a decreased number of extrasystoles in 2 FC by 39,9 percent and by 13,7 percent, respectively. On patients with 3 FC – by 38,7 percent (p<0,001) and by 13,3 percent (p<0,05), respectively. This effect could still be observed after two months after the treatment. The results showed, that laser therapy lessened extrasystoles and that it can be applied as an effective means when treating ventricular and supraventricular extrasystoles in patients with angina pectoris 1-3 FC.


T.M. Zenkovskaya, O.A. Matronchick, F.A. Khairullina, A.D. Kats

Hospital of War Invalids, Perm State Medical Academy, Russia

One can observe the impairment of endothelian vessels function (EVF) and rheological blood features (RhBF) in patients with various forms of ischemic heart disease. The task of this investigation is the study of infrared laser therapy (IRLT) influence to RhBF, EVF; the dependence of treatment effectiveness from above stated functions. 58 patients with stable angina pectoris III and IV functional class where under supervision. IRLT was performed by means of USOR and ASOR-2K apparatus with rate 300 Hz, the procedure lasted 10 minutes and it was given 10 times. To evaluate treatment effectiveness and its dependence on RhBF we have studied erythrocyte aggregation (EA), we have counted erythrocyte aggregation coefficient (EAC), the erythrocyte flowing (HF), it was marked by the index of flowing (IHF), spontaneous thrombocytcs aggregation (SThA) before and after IRLT and capillary fragility test (CFT). Before IRLT EAC was 0.78±0.04, after it was 0.89±0.06 (p<0.05), standard – 1.0. Before IRLT 1EF was 80,44±0.96%, after it was 90.47±0.91% (p<0.05). SThA was higher standard in the majority of patients before therapy. It decreased after the therapy. After CFT SThA reduced in patients given IRLT (25.81±1.50%, 20.39±0.91% and 17.51±0.11%). The lowering of SThA did not occur in patients treated only with medical preparations. The IRLT was effective in 91.7%. The effect of therapy was higher in patients with reliable lowering of EA, SThA, increased IEF. Therefore the IRLT is highly effective method of angina pectoris treatment and improves rheological properties of blood and endothelium vessels function.


G.W. Babushkina, I.M. Korochkin, A.V. Kartelishev

Bashkir Medical University, Ufa, Russian State Medical University, Moscow, RF

The improvement of treatment and rehabilitation of patients with CHD remains to be a vital problem in cardiology, as morbidity, disability and mortality rates keep steadily growing. However, insufficient efficacy of therapeutic methods, the development of retraction to antianginal drugs, their intolerance make medical men search for the new methods of CHD treatment, nonmedicamental ones inclusive. This paper presents the results of 10-year observation over two groups of patients with CHD (the main and control). 280 patients, who underwent a course of laser therapy (LT), have been included in the main group (A), the control (B) 155 patients have been treated conventionally. Prospective observation demonstrated new cases of acute myocardial infarction (AMI), mortality from CHD as well. There were 26 cases of AMI (II, 9 cases per 1000 population a year) in the main group, 27 AMI cases (27.4 per 1000 people a year) in the control over the observation period. The analysis of the acute attack and AMI development incidence in II – IV functional class (FC) stenocardia patients for 5 years before and after LT annual course demonstrated that the patients who underwent the repeated antirelapsing course of low intensity LT during remission showed reliable advantage (p<0,01 to p<0,05) of the method used as well as its prophylactic effect to prevent CHD progressing which was confirmed by biochemical blood analysis (lipid metabolism and rheologic properties). During the observation period there were 54 deaths: 8,9% in the main and 18,7% in the control group. CHD was the main cause of death in both groups which made 84% and 89.6% respectively of all death causes. Mortality rate was 17,4 and 32,6 respectively per 1000 population a year (p<0,01). On the whole, prospective analysis of the obtained data showed that LT course is directly dependent on its type and frequency of repeated courses and enables to prolong therapeutic remission of CHD by 2,5 times on the average. Thus, low intensity LT is a method of choice for patients suffering from different forms of angina pectoris and is carried out either in combination with conventional drug therapy or alone.

Cardiovasc Radiat Med. 1999 Jul-Sep;1(3):265-9.

Augmentation of the expression of proangiogenci genes in cardiomyocytes with low dose laser irradiation in vitro.

Khanna A, Shankar LR, Keelan MH, Kornowski R, Leon M, Moses J, Kipshidze N.

Medical College of Wisconsin, Milwaukee, USA.

BACKGROUND AND OBJECTIVE: Several reports suggest that low power red laser light (LPRLL) is capable of affecting cellular processes in the absence of significant thermal effect. The objective of the present study was to determine the effect of LPRLL on proliferation of fetal cardiomyocytes in vitro and on the expression of proangiogenic genes, transforming growth factor-beta (TGF-beta), and vascular endothelial growth factor (VEGF). STUDY DESIGN/MATERIALS AND METHODS: All cell cultures were irradiated with single-dose LPRLL using a He-Ne continuous wave laser (632 nm) with different doses. The effect of LPRLL on new DNA synthesis was studied by 3H thymidine-incorporation assay. VEGF and TGF-beta expression by cardiomyocytes was studied by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: We observed that a dose-dependent increase in cardiomyocytes proliferation can be obtained with LPRLL and that there is a significant increase in VEGF and TGF-beta mRNA expression by cardiomyocytes. CONCLUSIONS: These data may have significant importance leading to the establishment of new methods for myocardial photoangiogenesis and photoregeneration as well as in vitro proliferation of cardiac myocytes

Vopr Kurortol Fizioter Lech Fiz Kult. 1997 Sep-Oct;(5):9-11.


The dynamics of the clinico-functional indices in patients with ischemic heart disease under the influence of repeated courses of laser therapy

[Article in Russian]

Vasil’ev AP, Strel’tsova NN.

Repeated courses of laser therapy given to patients with ischemic heart disease, angina of effort class I-IV for 2 years brought about stabilization of coronary insufficiency and improvement of clinical and functional conditions. Microcirculatory picture of the bulbar conjunctiva, coronary reserve improved. The treatment had also a hypotensive effect.


Y.M. Kazakov, L.A. Zvyaginceva, et al.

Ukrainian medical stomatological academy, Poltava, Ukraine

The problem of rehabilitation of the ischemic heart disease (IHD) patients remains essential. Use of physical methods of treatment of the given category of the patients in connection with numerous lacks in drug treatment (collateral action, decrease of tolerance with long treatment, sometimes their inaccessibility for continuous treatment) gets the increasing distribution. 32 IHD patients are surveyed, from them 6 IHD patients had a combination with arterial hypertension. Average age explore patients – 66±4 years. On a background of a dietary nutrition and basic treatment with nitrates have carried out an irradiation of blood by the helium-neon laser through light-guide in cubital vein with capacity 1,5 mW, exposition – 30 minutes once per day within ten days. As a result of complex treatment there has come an improvement of a common condition of the patients was noted, normalization of rheological parameters of blood. Thus, intravenous laser blood irradiation can be used for the treatment of patients with IHD in stage of the ambulatory rehabilitation, including the patients of the senior age groups.


Y.L.Gabinsky, Y.R. Yakovlev, S.V. Yakovleva

Yekaterinburg Infarction Centre, Yekaterinburg, Russia

The results of treatment by low-intensity laser radiation of blood are given with taking into account of individual sensitivity in process of laser therapy. 600 patients with myocardial infarction and early angina pectoris (500 patients simultaneously with drug therapy were given laser therapy, 100 patients were given only drug therapy) were examined. The selecting of dose of laser radiation was based on refraction index of blood plasma in the field of laser radiation in vitro. Before the starting of laser therapy, in the middle of the course and at the end of it the state of health was estimated according to the theory of stress by Selie. In the group of patients, receiving laser therapy, the pronounced positive clinical effect has been registered. According to our data this effect reflects the changing of blood coagulation.


A.I. Koryakov

Sverdlovsk Regional Clinical Psychoneurological Hospital of the Disabled Soldiers, Yekaterinburg, Russia

The aim of this study was the comparison of antianginal effect of intravascular He-Ne laser irradiation of blood (ILIB) in patients with different functional classes of angina pectoris. The tolerance to exercise was studied in 44 men with stable angina, functional classes I-II have been registered in 20 patients, functional classes III-IV – in 24 patients. Four veloergometer tests both before and after the course of invasive treatment were performed for every patient under circumstances of total abolition drugs. All 44 patients were randomized by the blind method into ILIB and placebo groups. Nine men with the slight angina pectoris and 15 patients with the painful angina pectoris were subjected to 6 ILIB seances, the total course dose was 21,6±0.8 J. We used the He-Ne laser radiation (wavelength 632,8 nm) from the three-wave laser therapy device “Adept”, the light power in the end of intravenous light-guide was from I to 2 mW. Other 11 patients with angina functional classes I-II and 9 patients with angina pectoris functional classes III-IV received the placebo course of six invasive procedures. The effect of ILIB was calculated as a difference of increases in the mean-sample maximum workload between the laser therapy group and the placebo group. In patients with severe angina ILIB increased the exercise power reached on veloergometer on the average by 7,3±3,2 W (t = 5,26; p = 0). In contrast, in patients with the high tolerance to exercise ILIB did not significantly affect this parameter. We obtained the great difference in effects of ILIB in patients with the serious angina pectoris and the mild stenocardia (t = 2,41, p = 0,03), Thus, efficacy of the invasive blood irradiation with He-Ne laser in patients with stable effort angina pectoris depended on the initial tolerance to exercise.


A.I. Koryakov, N.N. Koryakova, A.I. Iofin

Sverdlovsk Regional Clinical Psychoneurological Hospital of the Disabled Soldiers, Yekaterinburg, Russia

We reported about the significant antianginal effect of intravenous He-Ne laser irradiation of blood (ILIB) in patients with serious stable effort angina functional classes 3-4. The goal of this work was to investigate an action of ILIB on an increase of the tolerance to exercise after the sublingual taking nitroglycerin in dose 0.5 mg in patients with stenocardia. We observed 27 men with stable effort angina functional classes II-IV. All patients were subjected two conjugate loading tests on veloergometer with nitroglycerin both before and after of the seven-day treatment. The antianginal effect of nitroglycerin was calculated as the magnitude of the increase maximum workload after taking nitroglycerin in comparison with the initial level of the maximum exercise power. Patients were divided randomly into main and control groups (17 and 10 men accordingly). Patients from the master group were subjected to six seances of ILIB. Power at the light-guide end was 1-2 mW, the accumulated dose was 21.6±0.8 J. The control group received the course of 6 invasive 45-minute placebo procedures that outwardly were like seances of ILIB. Both the intravascular laser treatment and its imitation were performed with three-wave laser therapy device “Adept”. In all patients invasive procedures were combined with antianginal medication treatment that was abolished 1 -2 days before loading tests. Although after the treatment the increase of antianginal effect of nitroglycerin was not statistically significant in both groups, nevertheless after ILIB the rise of nitroglycerin’s effect was more by 63 per cent as compared with placebo (p> 0.9). Thus, ILIB not only increases tolerance to exercise in patients with severe stable effort angina, but also it is not decreases the antianginal effect of nitroglycerin.

Vopr Kurortol Fizioter Lech Fiz Kult. 2002 Jul-Aug;(4):9-11.

Use of infrared laser therapy in patients with ischemic heart disease associated with diabetes mellitus type 2 in health resort

[Article in Russian]

Zin’kovskaia TM, Zavrazhnykh LA, Golubev AD.

Infrared laser therapy (300 Hz) combined with balneotherapy and patients’ education is more effective than standard sanatorium rehabilitation in patients with ischemic heart disease associated with diabetes mellitus type 2. 81.8% patients showed good response manifesting in less frequent anginal attacks, episodes of pain and painless ischemia and lower doses of antianginal drugs. Systolic and diastolic arterial pressure lowered by 18 and 10 mm Hg on the average, respectively. Multimodality rehabilitation of IHD patients with type 2 diabetes mellitus improves hemostasis, carbohydrate and lipid metabolism. Coronary circulation response lasted for 24 weeks.

Sov Med. 1990;(3):12-5.


Helium-neon laser therapy in the combined treatment of unstable stenocardia

[Article in Russian]

Korochkin IM, Kapustina GM, Babenko EV, Zhuravleva NIu.

He-Ne laser therapy included in complex of therapeutic methods for patients with unstable angina pectoris is a highly effective treatment modality; it helps essentially reduce the risk of acute myocardial infarction in these patients. Clinical efficacy of laser therapy is confirmed by its favorable action on hemostasis plasma factors, consisting in reduction of fibrinogen level, normalization of antithrombin-III (AT-III), decrease of the level of soluble fibrinomonomer complexes, this indicating a lowering of the blood coagulation potential. Absence of significant changes in plasminogen level may be an indicator of the nonenzymic route of fibrinogen system activation. Sessions of intravenous laser therapy should be administered 2-3 times a week to unstable angina pectoris patients with low AT-III levels, whereas for patients with initially high or normal AT-III levels combined laser therapy is advisable (4-5 daily invasive procedures and 6-8 skin surface ones on the Zakharyin-Head’s zones). Measurements of endogenic anticoagulants is an effective means for monitoring laser therapy in this patient population.

Vestn Khir Im I I Grek. 2000;159(2):60-4.

The effect of different methods of photohemotherapy on the rheological properties of the blood in patients with ischemic heart disease

[Article in Russian]

Gavrisheva IA, Dutkevich IG, Pleshakov VT, Kolesnik VS.

The authors made an analysis of results of examination of 41 patients with ischemic heart disease treated by the standard medicamentous therapy and when using different methods of photohemotherapy against its background. It was established that medicamentous therapy during 2 weeks failed to result in a substantial improvement of rheological properties of blood, while its combination with photohemotherapy could give a considerable positive effect coinciding with clinical improvement of the patient’s state. Shorter terms are required to correct hemorheological indices when autotransfusions of photomodified blood are used.

Vopr Kurortol Fizioter Lech Fiz Kult. 1995 Jan-Feb;(1):5-7.


The effect of different types of laser therapy on the reactivity of the peripheral blood neutrophils in patients with ischemic heart disease

[Article in Russian]

Siuch NI, Illarionov VE.

The responses to laser therapy (intravenous, continuous skin exposure without a magnet, magnetic laser therapy) of 83 patients with coronary heart disease aged 50-80 demonstrated the advantages of noninvasive laser irradiation of blood. Myeloperoxidase activity may serve a criterion for estimating the number of irradiation procedures needed.

Kardiologiia. 1993;33(2):22-3.


Changes in central hemodynamics and microcirculation during laser therapy in patients with coronary insufficiency

[Article in Russian]

Gel’fgat EB, Samedov RI, Kurbanova ZN, Gadzhiev GG.

The study was undertaken to examine 45 patients with Stages IIB-III heart failure (HF) by the classification developed by V. Kh. Vasilenko and N. D. Strazhesko. Thirty patients had laser therapy in addition to the routine treatment, 15 patients served as a control group. The combined drug treatment along with laser therapy in patients substantially improved peripheral circulatory parameters than in the controls. There was a positive dynamics of central hemodynamic parameters as shown by lower left ventricular volumes and higher myocardial contractile and pump functions. Improvement of microcirculatory and central hemodynamic parameters in patients treated with laser occurred in earlier periods of hospital stay than in the controls.

Vopr Kurortol Fizioter Lech Fiz Kult. 1996 Mar-Apr;(2):3-5. \


The laser therapy of patients with hypertension in combination with coronary insufficiency

[Article in Russian]

Kniazeva TA, Badtieva VA, Zubkova SM.

Hypertensive patients with coronary insufficiency have received infrared (lambda = 0.85 microns) laser radiation to the skin. The treatment is shown to have antianginal, antihypertensive effects, to improve cardiac performance, myocardial contractility, to increase myocardial, coronary and aerobic reserves. This clinicofunctional efficacy is accompanied by positive shifts in lipid metabolism, lipid peroxidation activity, antioxidant defense, hemocoagulation and microcirculation.

Vopr Kurortol Fizioter Lech Fiz Kult. 2005 May-Jun;(3):13-7.

Metabolic determinants of efficacy of infrared laser therapy in hypertensive patients with combined pathology

[Article in Russian]

Krysiuk OB, Ponomarenko GN, Obrezan AG, Kostin NA.

By a distinct pathogenetic direction of a therapeutic action of laser therapy (LT) on different regulators of blood pressure (BP) and metabolism, 109 patients with essential hypertension (EH) and atherosclerosis and/or diabetes mellitus were studied for LT efficacy depending on metabolis disorders. LT demonstrated metabolic neutrality and unefficacy in patients with multiple marked disorders of fat metabolism and hyperglycemia. Metabolic factors determining LT efficacy comprise hypercholesterinemia, hypertriglyceridemia and hyperglycemia. The factorial analysis points to essential factor restructuring in metabolic disorders. The obtained equation of multiple regression allows prognostication of the degree of a fall of mean BP in response to LT depending on the degree of metabolic disorders.

{Klin Med(Mosk). 2001; 79 (1): 41-44.






Efficiency of low-intensity laser radiation in essential hypertension.

Velizhanina I A, Gapon L I, Shabalina M S, Kamalova N N.

In a placebo-controlled study an antihypertensive activity of low- intensive laser radiation (LILR) was evaluated in 52 males with essential hypertension stage I. The placebo group consisted of 14 matched patients. LILR was used as monotherapy of 10 daily procedures. This treatment significantly lowered systolic, diastolic and mean arterial pressure. Moreover, diastolic arterial pressure did not rise high at submaximal bicycle exercise. Total peripheral vascular resistance also decreased. A good hypotensive effect was achieved in 90.4% cases. Thus, LILR is a highly effective treatment in essential hypertension stage I.

Vopr Kurortol Fizioter Lech Fiz Kult. 2001 Jul-Aug;(4):3-6.


The efficiency of low-intensity laser radiation in the treatment of arterial hypertension complicated by ischemic heart disease

[Article in Russian]

Shuvalova IN, Klimenko IT, Svinina NG, Tsereteli MV, Zankina VG, Miasoed FR.

The efficiency of low-intensity laser radiation (LILR) was studied in the treatment of 291 patients with arterial hypertension and ischemic heart disease. Clinical grounds are given for use of LILR red and infrared rays in rehabilitation of hypertensive patients with ischemia. The rehabilitation regimens can be differentiated according to the disease severity, type of hemodynamics, state of cerebral circulation.

Lik Sprava. 1998 Oct-Nov;(7):141-3.


The effect of low-intensity laser radiation in the infrared and red ranges on arterial pressure regulation in patients with borderline hypertension

[Article in Russian]

Shuvalova IN, Klimenko IT, Zhukova LP, Oborin IuI.

Effectiveness was studied of low-intensity laser irradiation on regulation of arterial blood pressure (BP) in 185 patients (51 men, 134 women). The above patients were prescribed four therapeutic complexes: group I was exposed to infra-red irradiation by zones; group II–to scanning Helium-Neon laser across the portal zone and paravertebrally CIII-Th5; group III–to helium-neon laser in the area of right sinocarotid zone; group IV underwent hydrolaser shower (in red and intra-red range). Complaints were studied as were data from laboratory investigations, the condition of different bodily systems, BP level, the functional state of the cardiovascular system as per electrocardiography and rheography findings. A positive clinical effect was achieved in all the groups studied. Employment of low-intensity laser irradiation in the rehabilitation of patients with borderline hypertension during the sanatorium stage was noted to strikingly enhance the efficiency of the therapy administered. It can be prescribed to patients irrespective of their hemodynamic types. Irradiation of the right sinocarotid zone and hydrolaser therapy are indicated to patients presenting with hypo- and eukinetic types of hemodynamics and baseline sympatheticotonia.

Lik Sprava. 1997 May-Jun;(3):110-1.


The effect of plasmapheresis and laser irradiation of the blood on the hemorheological and hemodynamic indices in hypertension patients

[Article in Russian]

Alizade IG, Karaeva NT.

Results are submitted of investigation designed to study effects of a combined use of plasmapheresis and laser irradiation of blood on hemorheologic and hemodynamic characteristics in 36 patients with stage II hypertensive disease. The course exposure of patients to a combined use of plasmapheresis and laser irradiation of blood led to a drop of arterial pressure in different hemodynamic groups at the expense of different parameters characterising the hemodynamic status. Thus combined use of plasmapheresis and laser irradiation of blood can be considered a promising nonmedicamentous therapeutic alternative in patients with hypertensive disease being associated with a drop of arterial blood pressure, and what is more, improvement in viscous- and elastic properties of blood as well as its hemodynamic indices.

Med Tr Prom Ekol. 1996;(8):10-2.


Effects of laser therapy on psychological parameters and arterial blood pressure in drivers with hypertension

[Article in Russian]

Umetov MA.

The study covered possibility to use laser therapy for correction of arterial hypertension in car and track drivers suffering from high blood pressure. Laser irradiation of infrared waves with wavelength of 0.89 micrometers appeared to have positive influence on the drivers facing arterial hypertension.

Lik Sprava. 1994 May-Jun;(5-6):29-32.


Experience in the use of autotransfusions of laser-irradiated blood in treating hypertension patients

[Article in Russian]

Alizade IG, Karaeva NT.

Autotransfusion of laser light-irradiated blood (5-7 sessions) was found to facilitate a steady arterial blood pressure fall by an average 24% of the initial level in patients with hypertensive disease. Drop in the arterial blood pressure following the course of autotransfusion of laser light-irradiated blood was accompanied by improvement in general condition of the patients, enhancement of the effectiveness of antihypertensive preparations, favourable shifts in immunological and haemorheological indices. After discharge from hospital beneficial clinical effect persisted for up to 4-8 months. The preliminary data obtained suggest that autotransfusion of laser light-irradiated blood may well be used as adjunct to a complex of therapeutic measures to be taken to control hypertensive disease.

Vopr Kurortol Fizioter Lech Fiz Kult. 1996 Mar-Apr;(2):3-5.

The laser therapy of patients with hypertension in combination with coronary insufficiency

[Article in Russian]

Kniazeva TA, Badtieva VA, Zubkova SM.

Hypertensive patients with coronary insufficiency have received infrared (lambda = 0.85 microns) laser radiation to the skin. The treatment is shown to have antianginal, antihypertensive effects, to improve cardiac performance, myocardial contractility, to increase myocardial, coronary and aerobic reserves. This clinicofunctional efficacy is accompanied by positive shifts in lipid metabolism, lipid peroxidation activity, antioxidant defense, hemocoagulation and microcirculation.

Gig Tr Prof Zabol. 1992;(4):32-3.


The correction of hemodynamic disorders with low-intensity infrared laser radiation in agricultural machine operators with borderline arterial hypertension

[Article in Russian]

Mokretsov VV, Utts SR.

Data on the influence of low-intensity infrared laser on the central and peripheral hemodynamics in 76 agricultural machine operators facing transitory arterial hypertension are presented. Analysis revealed that low-intensity laser is more effective at the early stages of cardiovascular diseases. Low-intensity infrared laser exposure of reflexogenic zones can be effectively used to correct hemodynamic disorders in subjects facing transitory arterial hypertension.

A possible mechanism of the hypotensive effect of laser irradiation in patients with ischemic heart disease with arterial hypertension

[Article in Russian]

Vasil’ev AP, Strel’tsova NN.

93 patients with ischemic heart disease, 44(47.3%) of them with arterial hypertension, were exposed to laser irradiation (LI). LI was accompanied with lowering of arterial pressure, more prominent in hypertensive patients. Mechanism of the hypotensive effect of laser therapy operates largely through stabilization of the lipid bilayer of the cell membrane demonstrated on the model of erythrocyte.

Vopr Kurortol Fizioter Lech Fiz Kult. 2001 Jan-Feb;(1):15-8.


Evaluation of the effectiveness of normobaric hypoxia and low-intensity laser radiation in hypertensive patients from 24-hour arterial pressure monitoring data

[Article in Russian]

Velizhanina IA, Evdokimova OV.

A randomized parallel study was performed to compare the antihypertensive effect of normobaric hypoxia and low energetic laser irradiation in 57 patients with essential hypertension stage I using 24-hour blood pressure monitoring. High hypotensive efficacy of both methods is demonstrated. A course of normobaric hypoxia decreased mean 24-h and mean daytime systolic and diastolic blood pressure. Low energetic laser irradiation reduced mean 24-h, mean daytime and mean night systolic and diastolic blood pressure.

Vopr Kurortol Fizioter Lech Fiz Kult. 1998 Jan-Feb;(1):9-11.


The laser therapy of hypertension patients in the initial stages

[Article in Russian]

Velizhanina IA, Shabalina MS, Gapon LI, Kamalova NN, Sergeichik OI.

The effect of low-energy laser irradiation used as monotherapy was studied in 42 patients with early essential hypertension. Hypotensive and antioxidant effects of laser therapy, its ability to decrease total peripheral resistance were more pronounced in patients with stage I hypertension.

Fiziol Zh. 2003;49(1):104-8.






Vopr Kurortol Fizioter Lech Fiz Kult. 2003 Mar-Apr;(2):7-10.

Laser-, ultraphono-, and acupuncture in complex treatment of patients with hypertension

[Article in Russian]

Sobetskii VV.

368 patients with hypertensive disease stage I and II were examined and treated either with laser puncture or acupuncture. Laser puncture was effective at stage I of hypertensive disease while acupuncture had a more potent hypotensive effect and can be used both in hypertensive disease stage I and II. Action on the acupuncture points and zones normalizes also parameters of the central and peripheral hemodynamics in hypertensive patients.

Vrach Delo. 1990 Jun;(6):19-21.


The effectiveness of laser puncture in hypertension patients

[Article in Russian]

Odud AM, Potapenko PI.

A study is presented of the results of laser puncture in combination with drug treatment of patients with hypertensive disease using an association of different acupuncture points. The values of hemodynamics were evaluated by routine techniques of tetrapolar chest rheo- and kinetocardiography. The use of laser puncture allowed to reduce the dose of hypotensive drugs.

Vrach Delo. 1991 Jul;(7):34-6.


The use of laser puncture for managing hypertensive crises

[Article in Russian]

Odud AM, Potapenko PI.

The authors report efficacy of using laser puncture and pointed massage in controlling hypertensive crises in patients suffering of hypertensive disease. The arterial pressure was reduced mainly due to decrease of the peripheral resistance. This method of treatment was more effective in patients with moderate and significant hypertrophy of the left ventricle as compared with patients showing marked hypertrophy of the left ventricle.

Kardiologiia. 1991 Feb;31(2):67-70.


Optimization of the treatment of hypertensive disease from the rheological viewpoint

[Article in Russian]

Shabanov VA, Kitaeva ND, Levin GIa, Karsakov VV, Kostrov VA.

The efficacy of various modes of correcting rheological disorders (membrane-protective agents, laser irradiation, plasmapheresis was compared in hypertensive patients. In 30% of the patients, the conventional antihypertensive therapy was demonstrated to deteriorate hemorheological parameters, which was due to its atherogenic impact on the blood lipid spectrum. Essential phospholipids, laser irradiation, and plasmapheresis, which are supplemented to the multimodality therapy promote a significant improvement of hemorheological parameters, which makes it possible to recommend them for management of hypertensive patients with a stable (essential phospholipids), complicated (laser irradiation), and refractory (plasmapheresis) course.

Ter Arkh. 2001;73(10):70-3.


Changes in blood rheological properties in patients with hypertension

[Article in Russian]

Shabanov VA, Terekhina EV, Kostrov VA.

AIM: To study hemorheology in patients with essential hypertension (EH), to improve EH treatment in terms of blood rheology. MATERIAL AND METHODS: Blood rheology, microcirculation, lipid plasm spectrum, central hemodynamics were studied in 90 patients with mild and 83 patients with moderate or severe EH as well as 30 healthy controls before and after treatment (hypotensive drugs, essential phospholipids, intravenous laser blood radiation, plasmapheresis). RESULTS: Hemorrheological disorders (subnormal deformability of the red cells and elastoviscosity of their membranes, disk-spherical transformation and hyperaggregation of blood cells, high dynamic viscosity) correlated with the disease severity, arterial pressure and total peripheral vascular resistance. Long-term (1-1.5 years) hypotensive therapy, especially with combination of beta-blockers with diuretics, has a negative effect on blood rheology. Optimisation of EH treatment in terms of blood rheology consists in using essential phospholipids in stable hypertension, intravenous laser radiation in complicated hypertension, plasmapheresis in drug-resistant hypertension. Such an approach not only significantly improves hemorheology but also provides good clinical and hypotensive effects in 75-80% patients. CONCLUSION: Blood viscodynamics should be taken into consideration in individual treatment of hypertensive patients.


Effect of infrared laser irradiation on the arterial blood pressure in liquidators of the accident at the Chernobyl power plan.

[Article in Ukrainian]

Korkushko OO.

Kiev Medical Institute UAPM.

Liquidators of Tchernobyl accident with discirculatory post-irradiation encephalopathy were treated with infra-red lazer irradiation together with a half doze of pharmacological agents usually used. Infra-red lazer irradiation has been shown to result in a significant reduce in the arterial pressure level, so it can be effective in correcting the disturbances in haemodynamics.

Sov Med. 1990;(3):21-3.


Intravenous laser irradiation of the blood in occlusive vascular diseases of the extremities

[Article in Russian]

Shval’b PG, Zakharchenko AIa, Sigaev AA, Kataev MI.

The authors analyze the results of clinical application of intravenous He-Ne laser irradiation of the blood in patients with obliterating diseases of the limb vessels. Starting from 1984, this method was employed in the treatment of 133 patients, of these 102 ones with atherosclerosis obliterans of the lower limb vessels, 17 with endarteritis obliterans, and 14 with Raynaud’s syndrome. Intravenous laser therapy proved to the most effective in atherosclerotic involvement of the vessels, when positive result was achieved in 77.5 percent of patients. The length of remission was up to 6 months. the method of treatment is described.



St. Petersburg , Chair of Balneology and Physical Therapy, Kirov Army Medical College
Success of hypertension treatment depends on how well the chosen treatment mode matches the patient’s genotype. Specialists of the Chair of Balneology and Physical Therapy (Kirov Army Medical College) have determined how the hypertensive patient’s genotype impacts the efficiency of magnetic-laser therapy.
Scientist: G.N. Ponomarenko , St. PetersburgFor additional information:
Photo, pictures:
Primary hypertension is one of the most frequent diseases, the treatment of which is still a problem to contemporary medicine. The disease is determined by some genes, each of the genes may exist in several variants. Respective combination of these genes forms the so-called “risk threshold” of disease origin. The major contribution to the hypertension evolution is made by the genes of angiotensinogen (AGT), angiotensin converting enzyme (ACE) and some others. Their combination determines not only the risk of disease occurrence, but also a potential success of its treatment. Thus, adrenolytic drugs act differently on central hemodynamics depending on the AGT and ??? genes’ polymorphism. Besides drug therapy, there exist physical treatment modes, specifically – magnetic-laser therapy. The St. Petersburg physicians have researched how its effectiveness depends on genotype.Magnetic-laser therapy effectiveness was evaluated with 101 patients with different variants of genes’ polymorphism. The polymorphism itself was determined with the help of the polymerase chain reaction, having taken some venous blood from the patients. Participants to the experiment underwent a radiation treatment course consisting of ten everyday sessions, the patients being examined before and after the course. Physicians watched the blood pressure changes within 24 hours and the reaction to physical activity (the patients were placed on the bicycle ergometer). Polymorphism of different genes determines the clinical behavior and treatment effectiveness to different extent. Magnetic-laser therapy helps the majority of hypertensive patients. The ??? gene has the highest influence on the magnetic-laser therapy results. In case of one variant of polymorphism (it is called MM- polymorphism) the blood pressure falls down to the greatest degree. The researchers have also found the combinations where the effect is the lowest (this is TT-polymorphism of the angiotensinogen gene). It is interesting to note that the variant of angiotensin converting enzyme (ACE) polymorphism is the worst for success of magnetic-laser therapy but it is optimal for drug treatment of high blood-pressure.There are several genes that determine effectiveness of hypertension treatment. To clearly perceive their joint impact it is necessary to continue the research. However, it is evident already which genes determine to the largest extent the blood pressure and the value the blood pressure can be lowered in this or that way. Probably the patients will be soon undergo genetic blood test first, and then, depending on its results, the treatment will be prescribed.***