Photomed Laser Surg. 2018 Apr;36(4):221-226. doi: 10.1089/pho.2017.4349.

Hemolasertherapy: A Novel Procedure for Gingival Papilla Regeneration-Case Report.

Zanin F1, Moreira MS2, Pedroni ACF3, Windlin M1, Brugnera AP4, Brugnera Júnior A5, Marques MM3.

Author information

1 Biophotonics Center at Institute Brugnera and Zanin-IBZ , São Paulo, Brazil .
2 Post Graduation Program, School of Dentistry, Ibirapuera University , Sao Paulo, Brazil .
3 Department of Restorative Dentistry of the School of Dentistry, University of Sao Paulo , Sao Paulo, Brazil .
4 PG student of Master Oral Laser Application, University of Liège , Liège, Belgium .
5 Associate Researcher of the National Institute of Science and Technology (INCT)-“Basic Optics and Applied to Life Sciences”-IFSC, University of Sao Paulo , Sao Paulo, Brazil .



Interdental papilla is of major importance to patients’ orofacial aesthetics, especially regarding anterior teeth as part of the smile’s harmony. Loss of gingival tissue, which constitutes interdental papilla, forms what in odontology is called black spaces. This loss, besides affecting the smile’s aesthetics, also provokes phonetic and functional damage.


The objective of the authors is to present the result of three clinical cases treated with an innovative technique called hemolasertherapy, which stimulates growth of gingival papilla and thus permanently fills in the black spaces.


The photobiomodulation therapy (PBMT) used a 660 nm diode laser (Laser Duo, MMO-São Carlos, SP, Brazil), punctual, contact mode in two steps: before the bleeding (first PBMT) and immediately after bleeding (second PBMT). Parameters used were power output: 100 mW, CW; diameter tip: 5 mm; spot area: 0.19 cm2; irradiation exposure time per point: 20 sec; 14 points per daily session; total of 2 sessions, with a 1-week interval; E: 2 J per point; E: per daily session, 28 J; irradiance per point: 0.52 W/cm2; fluence per point: 10.4 J/cm2. Total in two daily sessions: total energy: 56 J; total fluence: 294.75 J/cm, 560?sec total time. An in vitro preliminary study was simultaneously carried out to demonstrate what could happen at cellular level in hemotherapy clinical cases associated with PBMT laser application.


This initial study demonstrated that the blood clot originated from the bleeding provoked in the gingival area is rich in mesenchymal stem cells. PBMT enables preservation, viability, and further differentiation, stimulating the return of gingival stem cells, which would support their survival and differentiation in the blood clot, thus favoring interdental papilla regeneration.


Follow-up was done for a time span of 4-5 years and considered excellent with regard to papilla preservation.


dentistry; periodontology; photobiomodulation; stem cells