Impact of Photobiomodulation on T3/T4 Ratio and Quality of Life in Hashimoto Thyroiditis.
- Department of General Surgery, Bagcilar Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.
- Department of General Surgery, Bakirkoy Dr. Sadi Konuk Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.
- Department of General Surgery, Acibadem Taksim Hospital, Istanbul, Turkey.
- Department of General Surgery, Istanbul Kolan Hospital, Istanbul, Turkey.
- Department of General Surgery, Istanbul Medical Faculty, University of Istanbul, Istanbul, Turkey.
Objective and background: Hashimoto’s thyroiditis (HT) is both a B cell- and T cell-mediated, organ-specific autoimmune disease. No current treatment for underlying pathological mechanisms is available for HT and once diagnosed it requires long-term levothyroxine (LT4) treatment in most patients. The aim of our study was to evaluate the effects of photobiomodulation (PBM) on HT patients regarding thyroid functions, thyroid autoantibody levels, and decrease in hormone replacement needs.
Methods: A total of 350 patients, who were diagnosed with Hashimoto`s thyroiditis, were included in our study. Patients were classified into two groups. Group 1 (n=210) received PBM and dietary supplementation such as vitamin D, iron, and selenium. Group 2 (n=140) received dietary supplements only. Patients’ needs for LT4 replacement levels, triiodothyronine (T3), thyroxine (T4), and TSH levels, T3/T4 ratio, and thyroid autoantibody levels were evaluated.
Results: As we compare both groups, the increase in T3 levels and T3/T4 ratio was markedly superior in Group 1 (p=0.0001). The decrease in thyroid peroxidase antibody (TPO Ab) levels was also significantly different between both groups (p=0.0001). Hormone replacement needs were also significantly decreased in Group 1 compared with Group 2 (p=0.03). Low-level laser therapy (LLLT) is 70 times more effective in increasing T3/T4 ratio (p=?0.001) and 15 times more effective in decreasing levothyroxine dosage.
Conclusions: Our results are encouraging and PBM seems to be very effective in increasing T3/T4 ratio and decreasing TPO Ab levels and weekly dosages of LT4 replacement therapy. Anti-inflammatory properties of PBM are greatly responsible for these changes and PBM causes major improvements in HT-related symptoms of the patient.
Hashimoto thyroiditis; autoimmune thyroiditis; photobiomodulation
Effect of Three Different Protocols of Low-Level Laser Therapy on Thyroid Hormone Production After Dental Implant Placement in an Experimental Rabbit Model.
Abstract Objective: The purpose of this study was to assess the systemic effects of low-level laser therapy (LLLT) on thyroid gland function and, consequently, calcium regulation – as measured by serum triiodothyronine (T3), thyroxine (T4), and free calcium levels – when administered after dental implant placement in a rabbit model.
Background data: Protocols for the use of laser therapy in several clinical procedures are currently under investigation, as not all of the actions and systemic effects of laser irradiation have been clearly established.
Materials and methods: Forty male adult New Zealand rabbits were distributed across five groups of eight animals each: two control groups (C-I and C-II) of unirradiated animals, and three experimental groups (E-5, E-10, and E-20), each exposed to a distinct dose of gallium-aluminum-arsenide (GaAlAs) laser [?=830?nm, 50?mW, continuous wave (CW)] every 48?h for a total of seven sessions. The total dose per session was 5?J/cm2 in E-5, 10?J/cm2 in E-10, and 20?J/cm2 in E-20. Animals in C-II and all experimental groups underwent surgical extraction of the mandibular left incisor followed by immediate placement of an osseointegrated implant (Nanotite®, Biomet 3i™) into the socket. Animals in group C-I served as an absolute control for T3, T4, and calcium measurements. The level of significance was set at 5% (p?0.05).
Results: ANOVA with Tukey’s post-hoc test revealed significant differences in T3 and calcium levels among experimental groups, as well as significant within-group differences in T3, T4, and calcium levels over time.
Conclusions: Although not reaching abnormal values, LLLT applied to the mandible influenced thyroid function in this model.
Effects of Low–Level Laser Therapy on the Serum TGF-?1 Concentrations in Individuals with Autoimmune Thyroiditis.
- 11 Ultrasound Unit, Department of Radiology, Hospital das Clínicas, University of Sao Paulo Medical School , São Paulo, SP, Brazil .
Abstract Objective: The objective of this study was to evaluate the serum concentration of transforming growth factor-?1 (TGF-?1) after low–level lasertherapy (LLLT) in patients with hypothyroidism resulting from chronic autoimmune thyroiditis (CAT).
Background data: Certain data indicate that LLLT is effective in patients with hypothyroidism caused by CAT; however, the mechanisms of action of LLLT in thyroid tissue are unknown. Cytokines could play a role in the response to LLLT.
Methods: A randomized, placebo-controlled trial included 43 patients with a history of levothyroxine therapy for CAT-induced hypothyroidism. The patients were randomly assigned to receive either 10 sessions of LLLT (830?nm, 50?mW output power, and 707?J/cm2 fluence; L group, n=23) or 10 sessions of a placebo treatment (P group, n=20) twice a week. Levothyroxine was maintained at the same dose during the entire study period. TGF-?1 was measured both pre-intervention and 30 days post-intervention in both groups. The differences were calculated between the TGF-?1 values observed 30 days post-intervention and the pre-intervention TGF-?1 values for each group (intragroup).
Results: Comparing the differences in TGF-?1 levels between the L group (874.9±541.7?pg/mL) and the P group (-128.4±832.8?pg/mL) revealed that there was a statistically significant increase in TGF-?1 levels 30 days post-intervention in group L compared with the placebo group (p=0.0379).
Conclusions: This finding suggested that the significant increase in serum TGF-?1 levels in patients with CAT-induced hypothyroidism was associated with the thyroid LLLT procedure. Future studies of the effect of LLLT on TGF-?1 gene expression in thyroid tissue are necessary to confirm these findings.
Low-level laser in the treatment of patients with hypothyroidism induced by chronic autoimmune thyroiditis: a randomized, placebo-controlled clinical trial.
Chronic autoimmune thyroiditis (CAT) is the most common cause of acquired hypothyroidism, which requires lifelong levothyroxine replacement therapy. Currently, no effective therapy is available for CAT. Thus, the objective of this study was to evaluate the efficacy of low-level laser therapy (LLLT) in patients with CAT-induced hypothyroidism by testing thyroid function, thyroid peroxidase antibodies (TPOAb), thyroglobulin antibodies (TgAb), and ultrasonographic echogenicity. A randomized, placebo-controlled trial with a 9-month follow-up was conducted from 2006 to 2009. Forty-three patients with a history of levothyroxine therapy for CAT-induced hypothyroidism were randomly assigned to receive either 10 sessions of LLLT (830 nm, output power of 50 mW, and fluence of 707 J/cm(2); L group, n=23) or 10 sessions of a placebo treatment (P group, n=20). The levothyroxine was suspended 30 days after the LLLT or placebo procedures. Thyroid function was estimated by the levothyroxine dose required to achieve normal concentrations of T3, T4, free-T4 (fT4), and thyrotropin after 9 months of postlevothyroxine withdrawal. Autoimmunity was assessed by measuring the TPOAb and TgAb levels. A quantitative computerized echogenicity analysis was performed pre- and 30 days postintervention. The results showed a significant difference in the mean levothyroxine dose required to treat the hypothyroidism between the L group (38.59 ± 20.22 g/day) and the P group (106.88 ± 22.90 g/day, P<0.001). Lower TPOAb (P=0.043) and greater echogenicity (P<0.001) were also noted in the L group. No TgAb difference was observed. These findings suggest that LLLT was effective at improving thyroid function, promoting reduced TPOAb-mediated autoimmunity and increasing thyroid echogenicity in patients with CAT hypothyroidism.
ISRN Endocrinol. 2012; 2012: 126720.
Published online Dec 17, 2012. doi: 10.5402/2012/126720
Assessment of the Effects of Low-Level Laser Therapy on the Thyroid Vascularization of Patients with Autoimmune Hypothyroidism by Color Doppler Ultrasound
Background. Chronic autoimmune thyroiditis (CAT) frequently alters thyroid vascularization, likely as a result of the autoimmune process. Objective. To evaluate the effects of low-level laser therapy (LLLT) on the thyroid vascularization of patients with hypothyroidism induced by CAT using color Doppler ultrasound parameters.
Methods. In this randomized clinical trial, 43 patients who underwent levothyroxine replacement for CAT-induced hypothyroidism were randomly assigned to receive either 10 sessions of LLLT (L group, n = 23) or 10 sessions of a placebo treatment (P group, n = 20). Color Doppler ultrasounds were performed before and 30 days after interventions. To verify the vascularity of the thyroid parenchyma, power Doppler was performed. The systolic peak velocity (SPV) and resistance index (RI) in the superior (STA) and inferior thyroid arteries (ITAs) were measured by pulsed Doppler.
Results. The frequency of normal vascularization of the thyroid lobes observed in the postintervention power Doppler examination was significantly higher in the L than in the P group (P = 0.023). The pulsed Doppler examination revealed an increase in the SPV of the ITA in the L group compared with the P group (P = 0.016). No significant differences in the SPV of the STA and in the RI were found between the groups.
Conclusion. These results suggest that LLLT can ameliorate thyroid parenchyma vascularization and increase the SPV of the ITA of patients with hypothyroidism caused by CAT.
2. Material and Methods
Patient selection was carried out between March 2006 and January 2008 in the Thyroid Outpatient Clinic of the Hospital das Clínicas, University of Sao Paulo Medical School (HC-FMUSP). This study was conducted at the Radiology Institute of the HC-FMUSP, where most of the population is exposed to a sufficient intake of iodine .In the Thyroid Outpatient Clinic, 108 patients with established hypothyroidism caused by CAT who were most likely to be eligible for inclusion in the trial were initially selected. These patients were undergoing treatment with stable doses of at least 50?g/day levothyroxine (LT4).
2.2. Inclusion Criteria
Patients who were between the ages of 20 and 60 years old, who had been undergoing LT4 therapy for hypothyroidism that was induced by CAT, and who had normal serum levels of triiodothyronine (T3), tetraiodothyronine (T4), free-T4(fT4), and thyrotropin (TSH) were selected for the study. The appropriate dose of LT4 replacement for each patient was determined by the doctors of the Thyroid Outpatient Clinic.The CAT diagnosis was defined by the presence of the following criteria: (1) high serum levels of thyroid peroxidase antibodies (TPOAbs) and/or antithyroglobulin (TgAbs) antibodies (>100U/mL); (2) overt hypothyroidism that required LT4 replacement and (3) an ultrasound pattern compatible with CAT [14–16].
2.3. Exclusion Criteria
Exclusion criteria included: (1) the use of immunosuppressants, immunostimulants, and drugs that interfere with the production, transport, and metabolism of thyroid hormones (e.g., corticosteroids, lithium, and amiodarone); (2) CAT with normal thyroid function; (3) CAT with subclinical hypothyroidism; (4) thyroid nodules; (5) hypothyroidism stemming from postpartum thyroiditis (up to 18 months after gestation); (6) a history of Graves’ disease; (7) prior treatment with radioiodine; (8) tracheal stenosis; (9) pregnancy; (10) a history of exposure to ionizing radiation and/or neoplasia in the cervical area; (11) previous surgical intervention in the thyroid; (12) thyroid hypoplasia; (13) ectopic thyroid; (14) serious illness (e.g., cancer, ischemic coronary artery disease, stroke, and kidney or liver failure).Of the 108 patients who initially presented with a high probability of inclusion, 47 were subsequently excluded because they did not fully meet the eligibility criteria, 14 lacked the time or transportation to get to the hospital, and 4 declined to participate (Figure 1). In the end, 43 patients were included in the study.
HC-FMUSP’s Research Ethics Committee approved this study and the patient consent forms. All of the patients willingly signed the consent form.
2.4. Study Design
We designed a randomized, placebo-controlled clinical trial to evaluate the effects of LLLT on the thyroid glands of CAT patients by employing CDU variables. A computerized random number generator was used to individually assign patients to either the LLLT group (L) or the placebo group (P), and the resulting computer-generated codes corresponding to the group assignments were placed in individual opaque envelopes numbered from 1 to 60. The envelopes were only opened by the investigator responsible for the interventions (Höfling, DB) at the time of each patient’s first therapy procedure; this practice guaranteed that the investigator was unaware of the group to which each patient was assigned until the envelope was opened. This researcher became aware of the patient assignments only after the allocation of patients to each group because it was necessary to adjust the equipment to administer either LLLT or the placebo. Thus, except for this investigator and the statistician, all of the patients, the Thyroid Outpatient Clinic physicians (who adjusted the appropriate dose of LT4 replacement), the laboratory personnel, and the CDU researcher were blinded to the treatment assignments for the duration of the study.Ultrasound was used to mark the thyroid limits on the skin with a dermographic pen. Next, we created a mask (mold) for each patient using an adhesive plastic material that displayed the following demarcations: the gland contours (containing perforations of 3mm in diameter separated by 1cm on the inside), the jugular notch of the sternum, and the thyroid cartilage prominence; these latter 2 structures were easy to detect, which made the placement of the mask at the exact location of the thyroid simple and reproducible. The device tip was placed perpendicular to the skin in contact mode through the perforations located within the thyroid boundaries of the mold.Both groups underwent ten sessions of either LLLT or placebo treatment twice a week for 5 weeks (Figure 2). The laser equipment was calibrated before each procedure.
The L group was treated with a continuous-wave diode laser device (830nm, infrared) with a beam area of 0.002827cm2 (Theralase, DMC, São Carlos, SP, Brazil) using the punctual method in the continuous emission mode at an output power of 50mW and at a fluence of 707J/cm2 (40 seconds at each application point). Thus, the total energy deposited at each point was 2J. The P group was treated using the same method and equipment, except an ordinary red light that was indistinguishable from the laser beam that was used as a placebo; this approach blinded the patients to which treatment they received.
2.5. Biochemical Measurements
The serum levels of TSH, T3, T4, fT4, TPOAb, and TgAb were measured using AutoDELFIA kits (Wallac Oy and PerkinElmer, Turku, Finland) prior to the intervention (Figure 2). Serum TRAb levels were determined prior to treatment (Figure 2) using a radio-receptor assay (RSR, Cardiff, UK). The reference values, analytical sensitivities, intraassay coefficients of variations and inter-assay coefficients of variations for the aforementioned assays are the following, respectively: (1) T3 = 70–200ng/mL, 20ng/dL, 2.9% and 1.2%; (2) T4 = 4.5–12.0?g/dL, 0.39?g/dL, 2.7% and 1.4%; (3) fT4 = 0.7–1.5ng/dL, 0.16ng/dL, 1.2% and 3.7%; (4) TSH = 0.4–4.5?U/mL, 0.01?U/mL, 2.8% and 2.2%; (5) TPOAb < 35U/mL, 1.0U/mL, 3.8% and 4.8%; (6) TgAb < 35U/mL, 1.0U/mL, 3.1% and 8.8%; (7) TRAb < 8.0%, 8%, 6.8%, and 9.6%.
2.6. Color Doppler Ultrasound Study
All of the CDU examinations were performed by a single-blinded investigator. CDU was performed using an HDI-5000 device (Philips Medical Systems, Bothell, WA, USA) attached to a broadband linear probe (5–12MHz). CDU examinations were performed on patients who were using the same doses of LT4 at before intervention and 30 days after intervention (Figure 2).
2.7. Examination Technique
The CDU study was performed with the patient in the supine position and a cushion under their shoulders with their neck hyperextended. To avoid underestimating the vascularization intensity, the probe was lightly positioned on the skin without any compression. The CDU images were obtained using power and pulsed Doppler imaging.
2.8. Power Doppler
A short apnea was requested from the patients during the power Doppler recordings. The equipment was set to “thyroid” and configured as follows: pulse repetition frequency (PRF) between 700 and 1000Hz, Map 1, Med, wall filter (WF) and flow opt: Med V. The probe was positioned in the longitudinal direction with its center at the projection of the middle third of the thyroid lobes. The isthmus vascularization was not assessed. The color gain was adjusted up to the highest possible level that was not associated with artifacts of image saturation. All of the two-dimensional images were recorded at the time of greatest visible flow, corresponding to the systolic peak velocity of blood flow.For the power Doppler analysis, the parenchyma vascularization was divided into four categories. Pattern I: the vascularization is decreased and is limited to the main peripheral arteries, which have reduced signals (assigned value = 0; Figure 3(a)). Pattern II: the vascularization is limited to the main peripheral thyroid arteries, which exhibit the usual signals, whereas there is either no flow signal in the parenchyma or only signals of vascularization focal points with either a scattered distribution (assigned value = 1; Figure 3(b)). Pattern III: clearly increased vascularity with a scattered distribution (assigned value = 2; Figure 3(c)). Pattern IV: a marked increase in vascularization with a diffuse and homogeneous distribution, including the so-called “thyroid inferno” pattern  (assigned value = 3; Figure 3(d)).
We assigned vascularization patterns from I to IV to the right and left lobes of each patient’s thyroid, with 46 lobes in the L group and 40 lobes in the P group.
2.9. Pulsed Doppler
The sampling volume was set to 2mm. The PRF was set according to the speed of the flow and to the parameters that yielded the best possible graphic representation. The evaluation of the superior thyroid artery was accomplished with the probe positioned in the oblique sagittal plane, close to the superior thyroid pole. The inferior thyroid artery was examined in the oblique axial plane, close to the transition between the middle and the inferior third of the thyroid. For the evaluation of the inferior thyroid artery, the cursor was set close to the trachea to avoid artifacts coming from the common carotid artery and the internal jugular vein. The systolic peak velocity and resistance index (RI) values in the superior and inferior thyroid arteries were obtained from pulsed Doppler analysis. The Doppler angle was always corrected to values less than or equal to 60°. The mean of the values found in the right and left lobes was used as the representative parameter.
2.10. Statistical Analysis
The statistical analysis was conducted primarily using SPSS software (version 16.0—SPSS Inc., IBM Headquarters Company, Chicago, IL, USA). The results were compared by paired and unpaired Student’s t-tests for normally distributed data and Mann-Whitney tests for data that departed substantially from a normal distribution. Fisher’s exact tests were used to analyze the categorical data. The results of the continuous variables are presented as the means ± confidence interval (CI). Two-sided P values <0.05 were considered statistically significant (*).
Forty-three patients with hypothyroidism caused by CAT were enrolled in the study and randomized to receive either LLLT (L group, n = 23) or placebo treatment (P group, n = 20). No patients dropped out during the study period (Figure 1). No significant differences between the randomization arms were found in any of the key variables (Table 1).
All 43 randomized patients, underwent 10 sessions of either LLLT or placebo treatment, were subjected to CDU pre- and 30 days after intervention and were included in the final statistical analysis (Figure 1).
3.1. Color Doppler Ultrasound
Based on the preintervention power Doppler study, the thyroid vascularization was abnormal (increased or decreased) in 22/23 (95.65%) patients in the L group and in 17/20 (85.00%) patients in the P group. In the L group, 19/23 (82.61%) exhibited hypervascularization, 3/23 (13.04%) exhibited reduced vascularization, and only 1/23 (4.35%) exhibited normal vascularization of the thyroid parenchyma. In the P group, 14/20 (70.00%) exhibited hypervascularization, 3/20 (15.00%) exhibited reduced vascularization, and 3/20 (15.00%) exhibited normal vascularization of the thyroid parenchyma.The frequency of normal vascularization of the thyroid lobes observed in the postintervention CDU study was significantly higher in the L than in the P group (P = 0.023; Table 2).
The assessment of the numerical values attributed to the vascularization was performed by paired and unpaired analyses. The paired analysis of the power Doppler study in the L group showed no statistically significant difference pre- and post-LLLT for the average value attributed to the vascularization pattern. In this group, the average value approached 1, which corresponds to normal vascularization. In contrast, a statistically significant increase (P = 0.015) for the average value attributed to the vascularization pattern was found in the P group, which distanced itself from the normal value 1 (Table 3).
The unpaired analysis between groups indicated that the average value attributed to the vascularization pattern after intervention was significantly lower in the L group and closer to 1 (the normal value) compared with the P group (P = 0.033; Table 1).The pulsed Doppler analysis showed an increase in the systolic peak velocity of the inferior thyroid arteries in the L group compared to the P group (P = 0.016; Table 1), whereas the systolic peak velocity of the superior thyroid arteries was not significantly different between the groups. The mean RI also did not reveal significant differences between the L and P groups (Table 1). Figures ?Figures4,4, ?,5,5, and ?and66 present examples of the vascularization patterns observed pre- and postintervention.
CDU was used to evaluate the effects of LLLT on the vascularization parameters of the thyroid gland, which are often altered in patients with TCA.The LLLT demonstrated anti-inflammatory properties and the ability to regenerate biological tissues [11, 12, 18]. It was revealed that these effects of infrared lasers may be associated, at least in part, with changes in the gene expression and serum levels of several cytokines, such as TNF-?, IL-1?, IL-2, IL-6, IL-8, interferon-? (IFN-?) and TGF-? [18–21]. Elevated levels of proinflammatory cytokines, such as IFN-?, TNF-?, IL-2, and IL-6, as well as the reduction of TGF-? levels, may play a role in CAT pathogenesis [22–24]. There are reports suggesting that LLLT can improve thyroid function, reducing the intensity of the autoimmune process against the gland as well as regenerating the follicle structure [6, 7]. It is possible that such LLLT effects may be mediated by cytokine modulation .Parenchymal hypervascularization, which was observed in most of the patients in both the L and P groups prior to the intervention, is frequently found in CAT patients [8, 9]. The comparison of the vascularization pattern between the 2 groups after the intervention showed that the average value attributed to the vascularization was significantly lower in the L group, that is, closer to the normal value of 1 (Table 1). The opposite effect was observed in patients in the P group, that is, their values were significantly increased from the normal value. This result suggests that LLLT contributed to the improvement of abnormal vascularization (increased or decreased) in the vast majority of patients in the L group. Indeed, after intervention, the proportion of patients with normal vascularization was greater in the group that was subjected to LLLT.High levels of TSH and TRAb could increase vascularization by elevating the levels of vascular endothelial growth factors in goitrous hypothyroidism and Graves’ disease . However, the TRAb serum levels were undetectable in all of the patients, and the levothyroxine dose was maintained until the completion of the postintervention CDU to maintain normal serum TSH levels.It has been shown that the parenchymal thyroid vascularization is not the result of an increased production of thyroid hormones or TSH but rather of the autoimmune activity in the gland [8, 10, 25]. The increased autoimmune-induced thyroid vascularization could be explained, at least in part, by the increased serum levels of C-X-C motif chemokine 10 (CXCL-10), which is stimulated by the cytokine interferon-? (IFN-?), which is, in turn, involved in the recruitment of Th1 lymphocytes . Newly diagnosed autoimmune thyroiditis patients demonstrate increased serum CXCL-10 levels . A significantly higher serum level of CXCL-10 was also described in patients with increased thyroid vascularization . The presence of higher CXCL-10 serum levels is more common in patients with CAT than in healthy individuals, and even after the correction of hypothyroidism by levothyroxine, there is no significant reduction of CXCL-10 . This result suggests that the high CXCL-10 levels are associated with the intensity of the autoimmune process and not with hypothyroidism [27, 28].The increased thyroid vascularization observed in most patients in this study, even in those undergoing treatment with levothyroxine, supports the hypothesis that the increased vascularization that is often present in CAT is independent of the serum TSH  and may be associated, at least in part, with the increasing levels of chemokine CXCL-10 [8, 27]. Considering that LLLT effects have been reported on several cytokines, it is plausible that this treatment may mediate, among others, the action of CXCL-10.In individuals with no gland dysfunction, the SPV in the superior thyroid artery is significantly higher than that in the inferior thyroid arteries . In this study, the preintervention SPV of the superior thyroid arteries was slightly higher than that of the lower arteries for both groups, although this difference was not statistically significant. However, the present study evaluated a smaller sample of patients with CAT than the cited study .Before the intervention, the SPV and RI of the superior and inferior thyroid arteries were similar between the L and P groups. After the intervention, the SPV and RI of the superior thyroid arteries showed no significant differences between the two groups. In contrast, the inferior thyroid arteries showed a significant SPV increase in the L group compared with the P group. The RI of these arteries showed no significant difference between the two groups. This result cannot be attributed to high levels of TRAb and TSH or to an increase in thyroid hormones in the L group because no patients had detectable levels of TRAb, and there was no change in the levothyroxine replacement doses at the times of CDU execution, either before or after the intervention. In our pilot study , the increase in the SPV of the inferior arteries was close to statistical significance after LLLT. This outcome could be associated with the LLLT effects on chemokines or other cytokines and vascular growth factors related to gland vascularization. Further studies could investigate the possible effects of LLLT on the gene expression of cytokines in the thyroid tissue to clarify this result.The increased SPV in the thyroid arteries is common in Graves’ disease; however, patients with hypothyroidism caused by CAT (who receive treatment either with or without levothyroxine) can also exhibit this increase . It has also been demonstrated that increases in SPV exist in the inferior thyroid arteries, even in cases of subclinical thyroid dysfunction, independent of serum TSH levels and thyroid hormones .In summary, both the presence of diffuse parenchymal hypervascularization and the increase in SPV of the thyroid arteries, as assessed by pulsed Doppler ultrasound, can be found in patients with hypothyroidism caused by CAT . The results of the abovementioned studies [8, 10, 25] indicate that the intensity of the color signals and the speed of the blood flow are probably the results of autoimmune activity, which is at least partly associated with the action of cytokines.Thus, the results of this study suggest that LLLT was able to improve thyroid parenchyma vascularization, which is associated with the autoimmunity that is present in the glands of CAT patients. This improvement could be an indirect result of thyroid autoimmunity reduction. Moreover, an SPV increase in the inferior thyroid arteries was found in these patients, and we speculate that this result may be due to the action of LLLT on chemokines. The significance of this finding is, however, uncertain. It should be noted that this study evaluated the CDU parameters one month post-LLLT; therefore, it will be important to assess the duration of the LLLT results on the thyroid vascularization. Such findings corroborate those reported in our previous studies [6, 7], and the authors hope to encourage further studies on this subject.In conclusion, the study of the thyroid gland using CDU showed that LLLT improved gland vascularization and increased the systolic peak velocity of the inferior thyroid arteries in patients with CAT-induced hypothyroidism.
Conflict of Interests
1. Boukis MA, Koutras DA, Souvatzoglou A, et al. Thyroid hormone and immunological studies in endemic goiter. Journal of Clinical Endocrinology and Metabolism. 1983;57(4):859–862. [PubMed]
The influence of pulsed infrared laser radiation on the hormone production in the thymus (an experimental study).
Local irradiation with pulsed (1500 Hz) low-energy infrared laser light of the thymus and thyroid gland region caused well-apparent stimulation of alpha-1-thymosin production in the healthy animals and normalized its level in the stressed ones. Similar stimulation of alpha-1-timosine biosynthesis was observed in an experiment with direct laser irradiation of the cultured HTSC epitheliocytes from the human thymus.
Lasers Surg Med. 2010 Aug;42(6):589-96.
Low-level laser therapy in chronic autoimmune thyroiditis: a pilot study.
Höfling DB, Chavantes MC, Juliano AG, Cerri GG, Romão R, Yoshimura EM, Chammas MC.
Radiology Institute (InRad), Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, Avenida Dr. Enéas de Carvalho Aguiar, CEP 05403-001 São Paulo, Brazil. firstname.lastname@example.org
BACKGROUND AND OBJECTIVES: Chronic autoimmune thyroiditis (CAT) remains the most common cause of acquired hypothyroidism. There is currently no therapy that is capable of regenerating CAT-damaged thyroid tissue. The objective of this study was to gauge the value of applying low-level laser therapy (LLLT) in CAT patients based on both ultrasound studies (USs) and evaluations of thyroid function and thyroid autoantibodies.
STUDY DESIGN/MATERIALS AND METHODS: Fifteen patients who had hypothyroidism caused by CAT and were undergoing levothyroxine (LT4) treatment were selected to participate in the study. Patients received 10 applications of LLLT (830 nm, output power 50 mW) in continuous mode, twice a week, using either the punctual technique (8 patients) or the sweep technique (7 patients), with fluence in the range of 38-108 J/cm(2). USs were performed prior to and 30 days after LLLT. USs included a quantitative analysis of echogenicity through a gray-scale computerized histogram index (EI). Following the second ultrasound (30 days after LLLT), LT4 was discontinued in all patients and, if required, reintroduced. Triiodothyronine, thyroxine (T4), free T4, thyrotropin, thyroid peroxidase (TPOAb) and thyroglobulin (TgAb) antibodies levels were assessed before LLLT and then 1, 2, 3, 6, and 9 months after LT4 withdrawal.
RESULTS: We noted all patients’ reduced LT4 dosage needs, including 7 (47%) who did not require any LT4 through the 9-month follow-up. The LT4 dosage used pre-LLLT (96 +/- 22 microg/day) decreased in the 9th month of follow-up (38 +/- 23 microg/day; P < 0.0001). TPOAb levels also decreased (pre-LLLT = 982 +/- 530 U/ml, post-LLLT = 579 +/- 454 U/ml; P = 0.016). TgAb levels were not reduced, though we did observe a post-LLLT increase in the EI (pre-LLLT = 0.99 +/- 0.09, post-LLLT = 1.21 +/- 0.19; P = 0.001).
CONCLUSION: The preliminary results indicate that LLLT promotes the improvement of thyroid function, as patients experienced a decreased need for LT4, a reduction in TPOAb levels, and an increase in parenchymal echogenicity.
Photomed Laser Surg. 2005 Dec;23(6):567-70.
Evaluation of low intensity laser effects on the thyroid gland of male mice.
Azevedo LH, Aranha AC, Stolf SF, Eduardo Cde P, Vieira MM.
Department of Oral Pathology, School of Dentistry, University Hospital, University of São Paulo, SP, Brazil.
OBJECTIVE: The purpose of this study was to assess whether there were alterations in the thyroid hormone plasma levels under infrared laser irradiation, in the thyroid gland region.
BACKGROUND DATA: Studies have demonstrated that infrared laser can cause alterations in thyroid glands.
METHODS: Sixty-five albino male mice were used and assigned to five groups (n = 13), with differences in the times that they were sacrificed. Irradiation procedures consisted of an infrared diode laser emitting at 780 nm, at 4 J/cm(2) energy density, in contact mode, point manner. Blood was collected before irradiation (group 1), and then at 24 h (group 2), 48 h (group 3) and 72 h (group 4), and 1 week (group 5) after the third irradiation. The collected material was used for clinical analysis to evaluate the T(3) (triiodothyronine) and T(4) (thyroxin) hormones. Five animals were used for light microscopy analysis.
RESULTS: A statistically significant hormonal level alteration between the first day and 7 days after the last irradiation was found.
CONCLUSIONS: It was concluded that low-level laser therapy (LLLT) of the thyroid gland may affect the level of thyroidal hormones.
Histol. Histopathol. 14, 1067-1071 (1999)
A quantitative investigation of microvascular changes in the thyroid gland after infrared (IR) laser radiation
C. Parrado1, F. Carrillo de Albornoz2, L. Vidal1 and I. Pérez de Vargas1.
1Department of Normal and Pathological Morphology, Faculty of Medicine, University of Málaga, Málaga and2Department of Surgery, Faculty of Medicine, University of Cádiz, Cádiz, Spain
Offprint requests to: Prof. L. Vidal, Department of Normal and Pathological Morphology, Faculty of Medicine, University of Málaga, 29080 Málaga, Spain
Summary. We present an ultrastructural study of thyroid capillaries in which 50-day-old rats Wistar rats, were irradiated with an infrared (IR) laser, (total dose, 46.80 J/cm2), the tissue quantified 1 day after ending treatment and a quantitative capillary analysis carried out by light and electron microscopy. Light microscopy was used to calculate capillary volume density revealing a significant increase in the irradiated rats. The quantitative measurement of parameters by electron microscopy required a two stage analysis: Level I, Electron Microscopy (Magnification x5,000); and Level II, Electron Microscopy (Magnification x26,000). At Level I, the following parameters were measured in each capillary: capillary area, capillary diameter, luminal area, luminal diameter, endothelial area, nuclear area and mean endothelial thickness. At Level II, pinocytotic vesicle diameter and their numerical density in endothelial cells were evaluated. Electron microscopic analysis revealed an increased luminal area in the capillaries of the irradiated rats. They also presented a decrease in endothelial cell thickness and vesicular diameter and an increase in vesicle numerical density. This latter increase is indicative of presumptive changes in capillary permeability, but the possible functional significance of these morphological changes in the endothelial cells requires further investigation.
Probl Tuberk. 1998;(4):29-31.
Effects of low-intensity laser irradiation on hormonal responsiveness of patients with pulmonary tuberculosis
[Article in Russian]
Egorova IL, Maliev BM, Sorokina IA, Ermakova GI, Tarnueva OK.
To examine the impact of low-intensive laser radiation (LILR) on endocrine function in patients with pulmonary tuberculosis, the time course of blood levels of thyroid hormones, cortisol, and insulin was studied in 117 patients of whom 64 received complex treatment by using LILR. Pulmonary tuberculosis was ascertained to be characterized by a marked hormonal imbalance, including impaired metabolism of thyroid hormones, hypercorticism, altered pancreatic incretion, which negatively affects the efficiency of antituberculosis therapy. Intravenous and epicutaneous therapy promotes recovery of endocrine responsiveness and enhances the efficiency of treatment in patients with pulmonary tuberculosis.
EFFECTIVENESS OF USING LASEROTHERAPY IN THE TREATMENT OF AUTO-IMMUNE THYREOIDITIS
Ukrainian Scientific-Research Institute for Pharmakotherapy of Endocryne Diseases, Kharkov, Ukraine
During last 10 years quantity of chronic auto-immune thyreoiditis (AIT) patients increased more than 10 times. This causes the search of new alternative methods of treatment. New pathogenic method of treatment AIT, which includes low-energetic laser radiation of thyroid gland (TG), was developed in Surgical Department. Transcuta-neous radiation ofTG proection was done on 21 patients with the help of semiconductor apparatus “Mustang-BlO” (Russia), wavelength 0,89 [im, pulse regime. Energetically control was provided by automated system of integral and differential electropuncture diagnostics “Lotos”. Dynamic investigations, conducted during 7 months after laserotherapy, showed decreasing sizes of TG and lymphoid infiltration centres with almost all patients, normalisation of thyroid hormones level against decreasing of substitutive therapy doses. When studying cellular and humoral immunity was found normalisation of T-suppressors and T-active lymphocytes against authentic decreasing of antythyroid anti-bodies (anti-bodies index to thyreoglobulin lowered from 1,25±0,11 to 0,45±0,05, P<0,001, tomicrosomal antigen from 0,69±0,05 to 0,41±0,03, P< 0,01). Normalisation of cellular and humoral immunity in the patients organism and also stop of auto-immune aggression processes and stimulation of reparative regeneration processes in TG proves that the method developed by us is pathogenic and may be recommended for AIT treatment.
RESTORATION OF STRUCTURE AND FUNCTION OF THYROID GLAND AND TIMUS OF RABBITS WITH EXPERIMENTAL AUTOIMMUNE THYROIDITIS
I.Gopkalova Ukrainian Scientific-Research Institute for Pharmakothcrapy of Endocrync Diseases, Kharkov, Ukraine
With the aim to find out mechanism of low-intensive laser radiation (LILR) effect on auto-immune thyreoiditis (AIT) pathogcnesis we provided researches at 30 Shinshilla rabbits with experimental AIT (EAIT). Laser radiation was done by apparatus Mustang-BlO (Russia), wavelength 0,89 (J.m, power 10 W in pulse, frequency 80 Hz, exposition on thyroid gland (TG) proection for 30 sec., every day, during 7 days. Studying ofmorphofunctional state of TG rabbits with EAIT in 14 days after laser therapy showed normalization of Klein index, height of follicular epithelium and coefficient of functional activity restrain, and also practically all figures of main TG structural components relative volumes (RV). Thus lymphoid infiltration RV decreased from (10,1±1,2)% to (0,24±0,2)%, P< 0,001; stromes RV -from (15,0± 1,8)% to (10,2±1,1)%, P< 0,05; grease cells RV – from (6,8±0,5)% to (4,3±0,5)%, P<0,01. Simultaneously RV of follicular epithelium increased from (10,0±0,1)% to (21,5±2,8)%, P< 0,001, and RV ofinter-follicular epithelium increased from (4,2+0,5)% to (11,2±1,5)%, P< 0,001. RV of crust and brain substance increased authentically in timus up to the norm limits at the account of decreasing RV of grease cells from (28,6±2,3)% to (3,9±0,3)%, P<0,001. Density of lymphocytes in crust and brain substance increased authentically. Thus LILR exerts favorable influence on the leading links of AIT pathogencsis: it stops auto-immune aggression in TG and stimulates reparative regenerative processes by restitutivc type, against stop of involutive processes in timus and activation of lyniphocitopoesis.