Endocr Regul 2010 Oct;44(4):155-63.

Possible role of low level laser therapy on bone turnover in ovariectomized rats.

Saad A, Yamany ME, Abbas O, Yehia M.


Objective. The aim of this study was to assess the effect of low level laser therapy (LLLT) on bone turnover markers in ovariectomized rats. <br />

Methods. Thirty adult female albino rats were divided into three groups; Group 1: 10 sham- operated control rats; Group 2: 10 bilaterally ovariectomized rats (OVX); Group 3: 10 OVX rats exposed to LLLT. LLLT was applied on the neck and shaft of femur, five times per week for 8 weeks. The dose applied on each point was 1000 Hertz, 5 Watts for 30 seconds with a total dose of 15 mJoule/cm2. At the end of experiment, blood samples were collected and sera were separated for determination of serum calcium (Ca), inorganic phosphorus (Pi), osteocalcin and alkaline phosphatase (ALP). In addition, a 24 hour urine sample was also collected from each rat for the determination of urinary calcium, phosphorous and deoxypyridinoline (U-DPD)/creatinine. <br />

Results. Significant increase in serum Ca, Pi , ALP, osteocalcin and significant decrease in U-DPD/creatinine in LLLT exposed group was found as compared to the other two groups. Bone morphological findings revealed the increase in calcium deposition and alkaline phosphatase of femoral bones in LLLT exposed group as compared to sham-operated and OVX rats. The software image analysis showed increased osteoblast numbers, decreased osteoclast numbers and increased compact bone thickness in LLLT exposed group. Significant positive correlations was obtained between osteoblast numbers and serum Ca , Pi, ALP and osteocalcin in LLLT exposed group ,while a significant negative correlation was noticed with U-DPD.<br />

Conclusion. The use of LLLT was found effective in enhancing bone formation and decreasing bone resorption in the osteoporotic OVX rats. Further studies are necessary to investigate the effect of different parameters of LLLT as wave length, duration and also numbers of sessions. The potential use of LLLT in postmenopausal women with osteoporosis is needed to be verified.

Keywords: Ovariectomy – Laser – Calcium – Phosphorus – Alkaline phosphatase – Osteocalcin – Osteoporosis.

Vopr Kurortol Fizioter Lech Fiz Kult. 1996 Sep-Oct;(5):25-6

The effect of low-intensity infrared laser therapy on the endocrine function of patients with climacteric disorders.

[Article in Russian]

Kulikova NG, Illarionov VE, Orekhov KV.

The authors examined males and females aged 40-65 with menopausal disorders. Low-intensive infrared laser therapy produced a response both in males and females in menopause as evidenced by normalization of FSH, LH levels and elevation of sex steroid hormones.

Zh Nevropatol Psikhiatr Im S S Korsakova. 1994;94(4):20-3.

Hormonal disorders in women with prosopalgia in osteochondrosis of the cervical spine and the possible means for their correction.

[Article in Russian]

Kushlinskii NE, Grechko VE, Ivanov NA, Klimenko II, Sineva NA, Vodop’ianov NP, Khasan A.

Radioimmunoassay was employed to determine basal levels of LH, FSH, prolactin, total testosterone and 17 beta-estradiol in plasma from 52 pre- or postmenopausal women with prosopalgia consequent to cervical osteochondrosis. The measurements were made before and after laser treatment. Different from the control, basal levels of the hormones and the trends in their changes due to laser therapy depended on the patients’ age and the disease stage.