Arkh Patol. 2003 Nov-Dec;65(6):8-13.

Morphological diagnosis of the inhalation trauma and criteria of efficacy of the endobronchial laser therapy based on the biopsy material

[Article in Russian]

Galankina IE, Dement’eva IV, Pinchuk TP, Maiorov GA.

N. V. Sklifosovsky Research Institute of Emergency Care, 129010, Moscow.

The results of 424 biopsies obtained at fibrobronchoscopy (FBS) from 45 patients with inhalation trauma (IT) are presented. The standard and endoscopic laser treatment (ELT) have been used (24 and 21 patients, respectively). It is demonstrated that precise visual assessment of IT severity is possible under dynamic endoscopic observation only. Healing of mucous defects of the tracheo-bronchial tree was shorter in the ELT group. Morphologically, early and continuous ELT was not associated with signs of severe purulent inflammation and, therefore, produced no early additional, damage to the wall. This may explain accelerated formation of the granulation tissue at the ulcer bottom and epithelization at the margin areas.

Ultrastruct Pathol. 2000 May-Jun;24(3):183-9.

Ultrastucture of the blood and lymphatic capillaries of the respiratory tissue during inflammation and endobronchial laser therapy.

Polosukhin VV.

Laboratory of Ultrastructural Research, Russian Academy of Medical Sciences, Novosibirsk, Russia.vpolosuk@unmc.edu

For wide application of low-energy laser irradiation in the pulmonary clinic, study of the structural basis of the therapeutic effect is necessary. The aim of this research is to describe the structural changes of the blood and lymphatic capillaries in the respiratory tissues during inflammation and following laser biostimulation. Comparative ultrastructural study was carried out on 127 open respiratory biopsy specimens from 45 patients with infectious-destructive lung diseases. These patients were divided into two groups, depending on tactic of pre-operative therapy: patients treated by only traditional anti-inflammatory measures and patients receiving additional laser therapy. Heightened permeability of the blood capillary endotheliocytes was noted as the initial stage in the development of the inflammatory reaction. Intensification of the process of permeability is accompanied by interstitial edema, deformation of the interalveolar septa, and structural disorganization of alveolar epithelium cells. Local lesions of microcirculation result in tissue hypoxia and induce processes of fibrosis. Laser biostimulation promotes reversion of the inflammatory process and stabilizes fibroplastic processes. Basic principles of pathogenetic therapy were stated. It was shown that low-energy laser irradiation satisfies these requirements as an additional method in the therapy of destructive lung diseases.

Probl Tuberk. 1991;(6):26-9.

Effect of low-energy laser irradiation of bronchial mucosa on systemic and local immunity in patients with chronic bronchitis

[Article in Russian]

Ivaniuta OM, Chernushenko EF, Dzublik AIa, Tyshko NA, Naida IV, Kulik IV.

The effectiveness of endobronchial low-energy laser therapy was studied in 28 patients with chronic nonobstructive bronchitis concurrent with thinning of bronchial mucosa. The course of treatment made it possible to obtain positive dynamics of most parameters of immunologic reactivity in CNB patients. Systemic immunity parameters, except for the NST and concentration of circulating immune complexes, underwent essential normalization. Marked positive changes were found in the parameters characterizing functional activity of alveolar macrophages: there was a 2.5-fold increase in adhesive properties and over 1.3-fold increase in the percentage of phagocytes. The level of secretory IgA rose significantly (by 3.5 times). Hence, a manifested therapeutic effect of this therapeutic method is mainly associated with its immune-stimulating action.

Lasers Surg Med. 1995;16(2):184-8.

He-Ne laser therapy by fibroendoscopy in the mucosa of the equine upper airway.

Gomez-Villamandos RJ, Santisteban Valenzuela JM, Ruiz Calatrava I, Gomez-Villamandos JC, Avila Jurado I.

Department of Veterinary Clinical Pathology, Faculty of Veterinary Medicine, University of Córdoba, Spain.


BACKGROUND AND OBJECTIVE: A study was made of the effects of low-level laser irradiation on the cicatrization of superficial wounds in the pharyngeal mucosa of the horse. STUDY DESIGN/MATERIALS AND METHODS: Duplicate pharyngeal mucosal ulcers were induced in 12 Spanish horses with submucosal injection of sulfuric acid. A fibroendoscope and an He-Ne laser were used to treat one of the ulcers and the second ulcer in each animal was left untreated, serving as a control. Biopsy samples for the histological study were taken after the seventh laser therapy session from the two animals. RESULTS: Irradiated lesions cicatrized at 10.5 days and nonirradiated lesions cicatrized at 18.0 days. Statistical analysis revealed highly significant differences (P < 0.0001) between the irradiated and control groups. Histological study of the control samples showed epithelial coagulation necrosis, edema, and microthrombi. However, in samples from the irradiated lesions no inflammatory edema, numerous active fibroblasts, connective tissue, and intensive epithelial regeneration were observed. CONCLUSION: Macroscopic and microscopic results indicated that laser treatment accelerated the cicatrization. Irradiated lesions cicatrized faster than control. These results suggest beneficial effects of low-level laser irradiation therapy on equine pharyngeal ulcerative lesions.