Hormonal Response

Vopr Kurortol Fizioter Lech Fiz Kult. 2011 Jul-Aug;(4):39-42.

The influence of pulsed infrared laser radiation on the hormone production in the thymus (an experimental study).

[Article in Russian]
[No authors listed]


Local irradiation with pulsed (1500 Hz) low-energy infrared laser light of the thymus and thyroid gland region caused well-apparent stimulation of alpha-1-thymosin production in the healthy animals and normalized its level in the stressed ones. Similar stimulation of alpha-1-timosine biosynthesis was observed in an experiment with direct laser irradiation of the cultured HTSC epitheliocytes from the human thymus.

Endocr Regul.  2010 Oct;44(4):155-63.

Possible role of low level laser therapy on bone turnover in ovariectomized rats.

Saad A, El Yamany M, Abbas O, Yehia M.


Physiology Department, Medical Research Institute, Alexandria University, Egypt.



The aim of this study was to assess the effect of low level laser therapy (LLLT) on bone turnover markers in ovariectomized rats.


Thirty adult female albino rats were divided into three groups; Group 1: 10 sham- operated control rats; Group 2: 10 bilaterally ovariectomized rats (OVX); Group 3: 10 OVX rats exposed to LLLT. LLLT was applied on the neck and shaft of femur, five times per week for 8 weeks. The dose applied on each point was 1000 Hertz, 5 Watts for 30 seconds with a total dose of 15 mJoule/cm². At the end of experiment, blood samples were collected and sera were separated for determination of serum calcium (Ca), inorganic phosphorus (Pi), osteocalcin and alkaline phosphatase (ALP). In addition, a 24 hour urine sample was also collected from each rat for the determination of urinary calcium, phosphorous and deoxypyridinoline (U-DPD)/creatinine.


Significant increase in serum Ca, Pi , ALP, osteocalcin and significant decrease in U-DPD/creatinine in LLLT exposed group was found as compared to the other two groups. Bone morphological findings revealed the increase in calcium deposition and alkaline phosphatase of femoral bones in LLLT exposed group as compared to sham-operated and OVX rats. The software image analysis showed increased osteoblast numbers, decreased osteoclast numbers and increased compact bone thickness in LLLT exposed group. Significant positive correlations was obtained between osteoblast numbers and serum Ca , Pi, ALP and osteocalcin in LLLT exposed group ,while a significant negative correlation was noticed with U-DPD.


The use of LLLT was found effective in enhancing bone formation and decreasing bone resorption in the osteoporotic OVX rats. Further studies are necessary to investigate the effect of different parameters of LLLT as wave length, duration and also numbers of sessions. The potential use of LLLT in postmenopausal women with osteoporosis is needed to be verified.

Transplant Proc. 2009 Dec;41(10):4313-5.

Effect of low-level laser irradiation on in vitro function of pancreatic islets.

Irani S, Mohseni Salehi Monfared SS, Akbari-Kamrani M, Ostad SN, Abdollahi M, Larijani B.

Students’ Scientific Research Centre, Tehran University of Medical Science, Tehran 1411413137, Iran.

INTRODUCTION: Although islet isolation and transplantation techniques have improved extensively in recent years, the loss of healthy functional islets is one of the major obstacles in this enterprise. A biostimulatory effect of low-level laser irradiation has been proven on proliferation of some kinds of cells. The aim of this study was to evaluate the effect of low-level laser irradiation on the function of isolated rat pancreatic islets after 24 hours of preculture.

METHODS: Pancreatic islets isolated from male rats (250 to 300 g) were cultured for 24 hours in RPMI 1640 media. Groups of islets then received different energy densities (1, 3, 5 joules/cm(2) or silent) at 2 wavelengths (810 nm and 630 nm) using laser devices. Insulin concentrations in buffer media were measured as indices of islet function.

RESULTS: Irradiation of incubated islets with 830 nm low-level laser significantly increased insulin secretion after a glucose challenge test (P < .05). There was a significant increase in insulin secretion after irradiation with joules/cm(2) 630 nm energy density (P < .001).

CONCLUSION: These findings suggest that low-level laser irradiations improved islet cell function before transplantation.

Lasers Med Sci. 2008 Apr;23(2):211-5. Epub 2007 Jul 10.

Effects of laser irradiation on the release of basic fibroblast growth factor (bFGF), insulin like growth factor-1 (IGF-1), and receptor of IGF-1 (IGFBP3) from gingival fibroblasts.

Saygun I, Karacay S, Serdar M, Ural AU, Sencimen M, Kurtis B.

Department of Periodontology, Gulhane Military Medical Academy, Etlik, Ankara, 06018 Turkey. saygunisil@yahoo.com

Various studies have shown biostimulation effects of laser irradiation by producing metabolic changes within the cells. Little is known about the biological effect of laser irradiation on the oral tissues. Among the many physiological effects, it is important to recognize that low-level laser therapy (LLLT) may affect release of growth factors from fibroblasts. Therefore, the aim of the present study was to determine whether the laser irradiation can enhance the release of basic fibroblast growth factor (bFGF), insulin-like growth factor-1 (IGF-1), and receptor of IGF-1 (IGFBP3) from human gingival fibroblasts (HGF). The number of all samples in the study were 30, and the samples were randomly divided into three equal groups; In the first group (single dose group), HGF were irradiated with laser energy of 685 nm, for 140 s, 2 J/cm(2) for one time, and in the second group, energy at the same dose was applied for two consecutive days (double dose group). The third group served as nonirradiated control group. Proliferation, viability, and bFGF, IGF-1, IGFBP3 analysis of control and irradiated cultures were compared with each other. Both of the irradiated groups revealed higher proliferation and viability in comparison to the control group. Comparison of the single-dose group with the control group revealed statistically significant increases in bFGF (p < 0.01) and IGF-1 (p < 0.01), but IGFBP3 increased insignificantly (p > 0.05). When the double dose group was compared with the control group, significant increases were determined in all of the parameters (p < 0.01). In the comparison of the differences between the two irradiated groups (one dose and two doses), none of the parameters displayed any statistically significant difference (p > 0.05). In both of the laser groups, LLLT increased the cell proliferation and cell viability. The results of this study showed that LLLT increased the proliferation of HGF cells and release of bFGF, IGF-1, and IGFBP3 from these cells. LLLT may play an important role in periodontal wound healing and regeneration by enhancing the production of the growth factors.

Tanzan Health Res Bull. 2007 Sep;9(3):196-201.

Low intensity laser therapy is comparable to bromocriptine-evening primrose oil for the treatment of cyclical mastalgia in Egyptian females.

Saied GM, Kamel RM, Dessouki N.

Department of General Surgery, Faculty of Medicine, Cairo University, Egypt.

One of the successful treatments for cyclical mastalgia is bromocriptine evening primrose combination. A double blind study was applied on 80 patients with cyclical mastalgia. They were randomly divided into two groups (A and B). In group A, patients were treated by bromocriptine/evening primrose. To group B, LILT with specified dosimetry was applied, using a device that delivers He-Ne laser combined with 4 infra-red diode laser. Evaluation of treatment was both subjective (using VAS) and objective (studying the degree of drop in plasma cortisol level). The drop of plasma cortisol with treatment was studied using the student -t distribution. A good response was observed in the laser group in 82.5%, compared to 63.9% in the bromocriptine/evening primrose group. There was a significant deference before and after treatment in both groups (P<0.05). This difference was more for the drug treated group than for the laser treated group, but in the latter, it acted on a wider sector of patients. In conclusion, LILT is recommended as a new treatment modality for cyclical mastalgia.

Ter Arkh. 2006;78(3):39-40.

[Clinical efficacy of target low-intensity laser radiation on the adrenal projection region in patients with bronchial asthma]

[Article in Russian]

Nikitin AV, Titova LA.

AIM: To study efficacy of infrared laser radiation on the adrenal projection region in patients with bronchial asthma (BA).

MATERIAL AND METHODS: The adrenal region of BA patients was exposed to infrared laser radiation. The effect was stated after blood count, examination of the urine and sputum, blood biochemical tests, blood hydrocortisone assay.

RESULTS: Positive clinical response to laser therapy manifested with improvement of clinical, device and spirometric indices in BA patients. Blood levels of hydrocortisone normalized.

CONCLUSION: Infrared laser radiation of adrenal projection region reduced the number of asphyxia attacks, improved bronchial drainage, shortened the disease duration. The method is well tolerated, safe, easy to make and suggested for use in combined treatment of BA.

Photomed Laser Surg. 2005 Dec;23(6):567-70.

Evaluation of low intensity laser effects on the thyroid gland of male mice.

Azevedo LH, Aranha AC, Stolf SF, Eduardo Cde P, Vieira MM.

Department of Oral Pathology, School of Dentistry, University Hospital, University of São Paulo, SP, Brazil.

OBJECTIVE: The purpose of this study was to assess whether there were alterations in the thyroid hormone plasma levels under infrared laser irradiation, in the thyroid gland region.

BACKGROUND DATA: Studies have demonstrated that infrared laser can cause alterations in thyroid glands.

METHODS: Sixty-five albino male mice were used and assigned to five groups (n = 13), with differences in the times that they were sacrificed. Irradiation procedures consisted of an infrared diode laser emitting at 780 nm, at 4 J/cm(2) energy density, in contact mode, point manner. Blood was collected before irradiation (group 1), and then at 24 h (group 2), 48 h (group 3) and 72 h (group 4), and 1 week (group 5) after the third irradiation. The collected material was used for clinical analysis to evaluate the T(3) (triiodothyronine) and T(4) (thyroxin) hormones. Five animals were used for light microscopy analysis.

RESULTS: A statistically significant hormonal level alteration between the first day and 7 days after the last irradiation was found. CONCLUSIONS: It was concluded that low-level laser therapy (LLLT) of the thyroid gland may affect the level of thyroidal hormones.

Patol Fiziol Eksp Ter. 2004 Oct-Dec;(4):24-5.

[Effect of magnetolaser therapy on dynamics of bioamines, heparin in injured peritoneum and healing of this injury]

[Article in Russian]

Arsiutov VP, Ivanov LN, Arsiutov OV.

An experimental study on 62 guinea pigs was made to examine a magnetic-laser effect on the levels of histamin, serotonin, catecholamine, heparin and pathomorphology of wound healing after operations in the abdominal cavity. Magnetolaser therapy promotes fast normalization of the above levels, suppression of inflammatory-necrotic processes and stimulation of reparative and regenerative processes.

Med Pregl. 2003 Nov-Dec;56(11-12):516-20.

[Laser biostimulation in the treatment of pleurisy]

[Article in Serbian]

Milojevi? M, Kuruc V.

Institut za plu?ne bolesti, Sremska Kamenica, Medicinski fakultet, Novi Sad. drmilojevic@yahoo.com

INTRODUCTION: Low-intensity lasers have been utilized in medicine in two ways: for local stimulation and for stimulation of acupuncture points. Literature data reveal that this method has been indiscriminately applied in psychiatry, rheumatology, gynecology, dermatology, otorhinolaryngology, in diverse acute and chronic pains, inflammations, vascular disorders, angina pectoris, bronchial asthma. Most commonly reported clinical effects are analgesia, spasmolytic and anti-inflammatory effects, as well as faster wound and bone healing. MATERIAL AND METHODS: This prospective study analyses effects of laser biostimulation on patients with pleurisy. The analysis included 25 patients treated at the Institute of Lung Diseases in Sremska Kamenica during 2000, 2001 and 2002. Apart from conservative treatment, these patients were treated with laser biostimulation of acupuncture points and local region for ten days. During treatment, changes of present clinical signs, general symptoms, radiological findings, as well as changes of some relevant biochemical parameters were recorded. RESULTS: Results were compared with the control group which included the same number of patients, who differed from the examined group only by not being exposed to laser biostimulation. The examined group of patients with pleuritis presented with quicker resorption of pleural effusion, less pleural adhesions, more significant decrease of clinical symptoms, especially pain, as well as more significant increase of cortisol and immunoglobulin A and decrease of circulating immune complexes (CIC), leukocytes and sedimentation rate than the control group. DISCUSSION: Mechanisms of laser biostimulation in treatment of pleurisy were described in detail and the obtained results were correlated to those reported by other authors. CONCLUSION: 1. Patients with pleurisy undergoing laser stimulation presented with faster resorption of effusion and remission of the subjective symptoms, as well as significant decrease of biochemical acute inflammation parameters in the peripheral blood and therefore with faster recovery. 2. In patients with pleurisy laser treatment increases regenerative mechanisms of the pleural surface, thus decreasing the quantity of formed adhesions and resulting in better mobility of the diaphragm.

Klin Khir. 2000 Aug;(8):42-4.

[The change of biogenic amine blood level under the influence of the low intensity laser infrared radiation]

[Article in Russian]

Povazhnaia ES, Sokrut VN, Lykov AA, Povazhnaia SV, Peklun IV.

The dynamics of the biogenic amines level in the blood of patients after performance of hemorrhoidectomy was dependent on severity of an organism stress-reaction predicting the wound healing outcome. When the wound irradiation using the low-energy laser of infrared diapason is performed in patients with the lowered reactivity of organism the histamine and serotonin contents dynamics is the same as in the patients with normal reactivity.

Histol. Histopathol. 14, 1067-1071 (1999)

A quantitative investigation of microvascular changes in the thyroid gland after infrared (IR) laser radiation

C. Parrado1, F. Carrillo de Albornoz2, L. Vidal1 and I. Pérez de Vargas1.

1Department of Normal and Pathological Morphology, Faculty of Medicine, University of Málaga, Málaga and2Department of Surgery, Faculty of Medicine, University of Cádiz, Cádiz, Spain

Offprint requests to: Prof. L. Vidal, Department of Normal and Pathological Morphology, Faculty of Medicine, University of Málaga, 29080 Málaga, Spain

Summary. We present an ultrastructural study of thyroid capillaries in which 50-day-old rats Wistar rats, were irradiated with an infrared (IR) laser, (total dose, 46.80 J/cm2), the tissue quantified 1 day after ending treatment and a quantitative capillary analysis carried out by light and electron microscopy. Light microscopy was used to calculate capillary volume density revealing a significant increase in the irradiated rats. The quantitative measurement of parameters by electron microscopy required a two stage analysis: Level I, Electron Microscopy (Magnification x5,000); and Level II, Electron Microscopy (Magnification x26,000). At Level I, the following parameters were measured in each capillary: capillary area, capillary diameter, luminal area, luminal diameter, endothelial area, nuclear area and mean endothelial thickness. At Level II, pinocytotic vesicle diameter and their numerical density in endothelial cells were evaluated. Electron microscopic analysis revealed an increased luminal area in the capillaries of the irradiated rats. They also presented a decrease in endothelial cell thickness and vesicular diameter and an increase in vesicle numerical density. This latter increase is indicative of presumptive changes in capillary permeability, but the possible functional significance of these morphological changes in the endothelial cells requires further investigation.

Probl Tuberk. 1998;(4):29-31.

[Effects of low-intensity laser irradiation on hormonal responsiveness of patients with pulmonary tuberculosis]

[Article in Russian]

Egorova IL, Maliev BM, Sorokina IA, Ermakova GI, Tarnueva OK.

To examine the impact of low-intensive laser radiation (LILR) on endocrine function in patients with pulmonary tuberculosis, the time course of blood levels of thyroid hormones, cortisol, and insulin was studied in 117 patients of whom 64 received complex treatment by using LILR. Pulmonary tuberculosis was ascertained to be characterized by a marked hormonal imbalance, including impaired metabolism of thyroid hormones, hypercorticism, altered pancreatic incretion, which negatively affects the efficiency of antituberculosis therapy. Intravenous and epicutaneous therapy promotes recovery of endocrine responsiveness and enhances the efficiency of treatment in patients with pulmonary tuberculosis.

Vrach Delo. 1991 Jan;(1):24-7.

[Biogenic amines and cyclic nucleotides in the laser therapy of long-term nonhealing stomach ulcers]

[Article in Russian]

Loginov AS, Sokolova GN, Trubitsyna IE, Sokolova SV, Markin EP, Varvanina GG.

A study is presented of the effect of copper vapors laser therapy on the content of biogenic amines–serotonin and histamine and the state of adenylcyclase (AC) system (content of cAMP, cGMP and AC activity) at the edge of the gastric ulcer. Direct effect of laser radiation (single dose 10–15 J.) produced a significant increase of serotonin, histamine, cAMP, AC activity and an insignificant increase of cGMP. Healing of the ulcerative defect after 5–6 laser therapy sessions was followed by a reduction of the content of serotonin, increase of histamine, cAMP and AC activity. The authors discuss the biostimulating effect of laser radiation by influencing the inflammatory-proliferative processes in the epitheliocytes in prolonged nonhealing gastric ulcers.


 J. Karachentsev

Ukrainian Scientific-Research Institute for Pharmakotherapy of Endocryne Diseases, Kharkov, Ukraine

During last 10 years quantity of chronic auto-immune thyreoiditis (AIT) patients increased more than 10 times. This causes the search of new alternative methods of treatment. New pathogenic method of treatment AIT, which includes low-energetic laser radiation of thyroid gland (TG), was developed in Surgical Department. Transcuta-neous radiation ofTG proection was done on 21 patients with the help of semiconductor apparatus “Mustang-BlO” (Russia), wavelength 0,89 [im, pulse regime. Energetically control was provided by automated system of integral and differential electropuncture diagnostics “Lotos”. Dynamic investigations, conducted during 7 months after laserotherapy, showed decreasing sizes of TG and lymphoid infiltration centres with almost all patients, normalisation of thyroid hormones level against decreasing of substitutive therapy doses. When studying cellular and humoral immunity was found normalisation of T-suppressors and T-active lymphocytes against authentic decreasing of antythyroid anti-bodies (anti-bodies index to thyreoglobulin lowered from 1,25±0,11 to 0,45±0,05, P<0,001, tomicrosomal antigen from 0,69±0,05 to 0,41±0,03, P< 0,01). Normalisation of cellular and humoral immunity in the patients organism and also stop of auto-immune aggression processes and stimulation of reparative regeneration processes in TG proves that the method developed by us is pathogenic and may be recommended for AIT treatment.

Folia Histochem Cytochem (Krakow). 1983;21(2):87-92.

Effect of low intensity laser beam on steroid dehydrogenase activity and steroid hormone production in cultured porcine granulosa cells.

Gregoraszczuk E, Dobrowolski JW, Galas J.

Effect of the beam emitted by a helium-neon (He-Ne) laser of a low power (2.8 mW) on the activity of delta 5, 3 beta-hydroxysteroid dehydrogenase (delta 5, 3 beta-HSD), as well as on the estradiol and progesterone production was studied in cultured granulosa cells obtained from porcine ovary. It has been found that the laser beam stimulates the activity of steroid dehydrogenase, increases estrogen levels and exerts a variable influence on the progesterone level. In control cultures the highest level of both hormones occurred simultaneously, while in cultures exposed to the laser beam progesterone level rose at a slower rate and reached maximum when the estrogen level dropped. Hyperplasia and epithelisation were observed only in the experimental cultures. The most intense cytochemical reaction for delta 5, 3 beta-HSD, as well as the most pronounced increase in estrogen and progesterone level occurred in cells exposed to a pulsating beam for 60 sec.

Vopr Kurortol Fizioter Lech Fiz Kult. 1996 Sep-Oct;(5):25-6

The effect of low-intensity infrared laser therapy on the endocrine function of patients with climacteric disorders

 [Article in Russian]

Kulikova NG, Illarionov VE, Orekhov KV.

The authors examined males and females aged 40-65 with menopausal disorders. Low-intensive infrared laser therapy produced a response both in males and females in menopause as evidenced by normalization of FSH, LH levels and elevation of sex steroid hormones.


Zh Nevropatol Psikhiatr Im S S Korsakova. 1994;94(4):20-3.


Hormonal disorders in women with prosopalgia in osteochoncrosis of the cervical spine and the possible means for their correction

[Article in Russian]

Kushlinskii NE, Grechko VE, Ivanov NA, Klimenko II, Sineva NA, Vodop’ianov NP, Khasan A.

Radioimmunoassay was employed to determine basal levels of LH, FSH, prolactin, total testosterone and 17 beta-estradiol in plasma from 52 pre- or postmenopausal women with prosopalgia consequent to cervical osteochondrosis. The measurements were made before and after laser treatment. Different from the control, basal levels of the hormones and the trends in their changes due to laser therapy depended on the patients’ age and the disease stage.


I.Gopkalova Ukrainian Scientific-Research Institute for Pharmakothcrapy of Endocrync Diseases, Kharkov, Ukraine

With the aim to find out mechanism of low-intensive laser radiation (LILR) effect on auto-immune thyreoiditis (AIT) pathogcnesis we provided researches at 30 Shinshilla rabbits with experimental AIT (EAIT). Laser radiation was done by apparatus Mustang-BlO (Russia), wavelength 0,89 (J.m, power 10 W in pulse, frequency 80 Hz, exposition on thyroid gland (TG) proection for 30 sec., every day, during 7 days. Studying ofmorphofunctional state of TG rabbits with EAIT in 14 days after laser therapy showed normalization of Klein index, height of follicular epithelium and coefficient of functional activity restrain, and also practically all figures of main TG structural components relative volumes (RV). Thus lymphoid infiltration RV decreased from (10,1±1,2)% to (0,24±0,2)%, P< 0,001; stromes RV -from (15,0± 1,8)% to (10,2±1,1)%, P< 0,05; grease cells RV – from (6,8±0,5)% to (4,3±0,5)%, P<0,01. Simultaneously RV of follicular epithelium increased from (10,0±0,1)% to (21,5±2,8)%, P< 0,001, and RV ofinter-follicular epithelium increased from (4,2+0,5)% to (11,2±1,5)%, P< 0,001. RV of crust and brain substance increased authentically in timus up to the norm limits at the account of decreasing RV of grease cells from (28,6±2,3)% to (3,9±0,3)%, P<0,001. Density of lymphocytes in crust and brain substance increased authentically. Thus LILR exerts favorable influence on the leading links of AIT pathogencsis: it stops auto-immune aggression in TG and stimulates reparative regenerative processes by restitutivc type, against stop of involutive processes in timus and activation of lyniphocitopoesis.