Pancreatic Function – Effects on

Transplant Proc. 2009 Dec;41(10):4313-5.

Effect of low-level laser irradiation on in vitro function of pancreatic islets.

Irani S, Mohseni Salehi Monfared SS, Akbari-Kamrani M, Ostad SN, Abdollahi M, Larijani B.

Students’ Scientific Research Centre, Tehran University of Medical Science, Tehran 1411413137, Iran.

INTRODUCTION: Although islet isolation and transplantation techniques have improved extensively in recent years, the loss of healthy functional islets is one of the major obstacles in this enterprise. A biostimulatory effect of low-level laser irradiation has been proven on proliferation of some kinds of cells. The aim of this study was to evaluate the effect of low-level laser irradiation on the function of isolated rat pancreatic islets after 24 hours of preculture. METHODS: Pancreatic islets isolated from male rats (250 to 300 g) were cultured for 24 hours in RPMI 1640 media. Groups of islets then received different energy densities (1, 3, 5 joules/cm(2) or silent) at 2 wavelengths (810 nm and 630 nm) using laser devices. Insulin concentrations in buffer media were measured as indices of islet function. RESULTS: Irradiation of incubated islets with 830 nm low-level laser significantly increased insulin secretion after a glucose challenge test (P < .05). There was a significant increase in insulin secretion after irradiation with joules/cm(2) 630 nm energy density (P < .001). CONCLUSION: These findings suggest that low-level laser irradiations improved islet cell function before transplantation.

Therapy of patients with chronic pancreatitis of alcoholic etiology by dalagrin and laser therapy of the blood.

[Article in Russian]

Bulychev VF, Vakhrushev IaM.

Effectiveness of dalargin and intravenous laser blood beaming (ILBB) in combined treatment of chronic alcoholic pancreatitis (CAP) was studied in 105 patients (8 females and 97 males) with CAP duration 1 to 25 years. Pancreatic function and treatment effects were studied by routine clinical investigations, advanced laboratory, biochemical tests, radioimmunossays. Pancreatic disorders in alcoholics present with high blood levels of trypsin and lipase, low levels of insulin and C-peptide. Serum concentrations of hydrocortisone and gastrin were elevated. Combined treatment of CAP with adjuvant dalargin and ILBB not only relieves clinical symptoms but also promotes normalization of pancreatic function.